Determination of Bactericidal Activity of Antibacterial Monomer MDPB by a Viability Staining Method
| Autor: | John F. McCabe, Kaoru Ohmori, Roy R. B. Russell, Nobuko Maeda, Satoshi Imazato, Yasuko Momoi |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Time Factors
Chromatography Dose-Response Relationship Drug biology Chemistry Colony Count Microbial Pyridinium Compounds biology.organism_classification Streptococcus mutans Anti-Bacterial Agents Microbiology Staining Dental Materials Viability staining chemistry.chemical_compound Monomer Microscopy Fluorescence Materials Testing Ceramics and Composites Fluorescence microscope Humans General Dentistry Fluorescence staining Fluorescent Dyes |
| Zdroj: | Dental Materials Journal. 27:145-148 |
| ISSN: | 1881-1361 0287-4547 |
| Popis: | In this study, the bactericidal activity of antibacterial monomer MDPB (12-methacryloyloxydodecylpyridinium bromide) against Streptococcus mutans was tested by a rapid method for monitoring viability. To S. mutans culture containing fluorescence staining solution that distinguishes live from dead cells, MDPB was added at a concentration of 250, 100, 50, or 10 microg/ml. Bacterial cells were observed by fluorescence microscopy and the percentage of dead cells was calculated. After 10, 20, or 30 minutes' contact with MDPB, the live/dead ratio was measured by fluorometry and viable counts (CFU) determined by the conventional plating method. Viability staining revealed that MDPB exhibited significant bactericidal effects at 50 microg/ml or greater (ANOVA, Fisher's PLSD test), and complete killing of the cells at 250 microg/ml of MDPB was demonstrated in conjunction with a plating method. The staining method thus provided a sensitive means to determine loss of viability, and indicated the strong killing effects of MDPB on S. mutans. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|