Effects of Percutaneous Collagen Induction Therapy Associated with Hyaluronic Acid on Inflammatory Response, Oxidative Stress, and Collagen Production
| Autor: | João Vitor Silvano Bittencourt, Paulo Cesar Lock Silveira, Rubya Pereira Zaccaron, Thiago Antônio Moretti de Andrade, Daniela Pacheco Dos Santos Haupenthal, Jennyffer Ione de Souza Silva, Samara de Sousa Mariano, Laura de Roch Casagrande, Germano Duarte Porto, Ligia Milanez Venturini, Carolini Mendes, Maria Eduarda Anastácio Borges Corrêa |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Male Antioxidant medicine.medical_treatment Immunology Inflammation Pharmacology medicine.disease_cause Fibroblast growth factor Antioxidants 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Percutaneous Coronary Intervention Hyaluronic acid medicine Immunology and Allergy Animals cardiovascular diseases Hyaluronic Acid Rats Wistar Wound Healing business.industry Induction Chemotherapy Fibroblasts Rats Interleukin 10 Oxidative Stress surgical procedures operative 030104 developmental biology chemistry 030220 oncology & carcinogenesis Conventional PCI Cytokines Tumor necrosis factor alpha Collagen medicine.symptom business Reactive Oxygen Species therapeutics Oxidative stress |
| Zdroj: | Inflammation. 43(6) |
| ISSN: | 1573-2576 |
| Popis: | Percutaneous collagen induction (PCI) is an alternative treatment for skin dysfunctions, it aims to stimulate collagen production by encouraging normal wound healing that occurs after any trauma by inducing microlesions; also it may be potentiated with the association with drugs such as hyaluronic acid (HA). Our objective was to evaluate the effects of PCI associated with hyaluronic acid (0.9%) on inflammatory process, oxidative stress, and collagen production in rat epidermis. For the study, 36 adult Wistar rats were randomly divided into 6 groups (n = 6): Control; PCI 0.5; PCI 1.0; HA; PCI 0.5 + HA; and PCI 1.0 + HA. The animals were anesthetized, trichotomized, and the application of therapies was performed once; After 7 days, the animals were euthanized for removal of the skin region. Levels of pro-inflammatory (IL1, IL6, TNFα), anti-inflammatory (IL4 and IL10) cytokines and growth factors (FGF, TGFβ) were evaluated, besides oxidative stress parameters and histological analysis. In combination groups, there is a decrease in TNFα compared with the control and PCI groups in contrast to a significant increase in anti-inflammatory cytokines and growth factors. Oxidant and oxidative damage levels showed a significant decrease in PCI + HA groups in relation to PCI groups while antioxidant defense increased in PCI + HA groups compared with the control group. The number of fibroblasts was increased in the PCI 1.0 group in relation to the control, HA, and PCI 0.5. The number of blood vessels and collagen area was increased in groups PCI and PCI + HA compared with the HA group. We conclude that the combination of PCI with HA is able to accelerate the acute inflammatory process, reducing its deleterious effects and anticipating the chronic response, contributing to tissue repair. |
| Databáze: | OpenAIRE |
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