Interleukin-10 and Interferon Gamma Gene Polymorphisms and Hepatitis C Virus-Related Liver Cirrhosis Risk
| Autor: | Hoda M. Moghazy, Abeer Sheneef, Aida A. Mahmoud, Asmaa N. Mohammad, Amal K. Noureldin, Mamdouh M. Esmat |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Adult Liver Cirrhosis Male Risk Cirrhosis Genotype Hepatitis C virus Immunology Hepacivirus medicine.disease_cause Polymorphism Single Nucleotide 03 medical and health sciences Interferon-gamma 0302 clinical medicine Gene Frequency Virology medicine Humans Interferon gamma Genetic Predisposition to Disease Genotyping Alleles business.industry Cell Biology Middle Aged Viral Load medicine.disease Hepatitis C Interleukin-10 Interleukin 10 030104 developmental biology Case-Control Studies 030211 gastroenterology & hepatology Female Gene polymorphism Restriction fragment length polymorphism business medicine.drug |
| Zdroj: | Journal of interferoncytokine research : the official journal of the International Society for Interferon and Cytokine Research. 37(4) |
| ISSN: | 1557-7465 |
| Popis: | The aim of the study was to evaluate the association between the gene polymorphisms in interleukin-10 (IL-10) and interferon gamma (IFN-γ) genes with susceptibility and severity of hepatitis C virus (HCV) infection among Egyptian patients. Interleukin-10 -592 A/C, -1082 G/A and IFN-γ +874 T/A genotypes were determined in 100 chronic HCV patients and 50 healthy controls using restriction fragment length polymorphism (RFLP) and the amplification refractory mutation system-polymerase chain reaction (ARMS-PCR) respectively. IL-10 -592 A/C polymorphism genotyping revealed that the frequency of CC genotype was significantly higher in chronic HCV patients than in controls (58% versus 30%, P 0.05). Regarding IL-10 -1082 G/A polymorphism genotyping, a higher frequency of GG genotype was found in chronic HCV patients compared to controls (31% versus 10%, P 0.05). IFN-γ +874 T/A genotyping showed that TT genotype was significantly higher in chronic HCV participants than controls (31% versus 18%, P 0.05), while a higher frequency of T allele was found in cirrhotic patients compared to noncirrhotic patients (P 0.05). Our observations suggested that IL-10 -592 A/C, -1082 G/A, and IFN-γ +874 T/A polymorphisms had a strong association with susceptibility to HCV infection. However, no significant association was observed between the cytokines (IL-10 and IFN-γ) genotypes profile and HCV-liver cirrhosis risk in the studied population, except for the high frequency of IFN-γ +874 T allele in cirrhotic patients. |
| Databáze: | OpenAIRE |
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