Lack of effect of the novel anticonvulsant SB-204269 on voltage-dependent currents in neurones cultured from rat hippocampus
| Autor: | Christopher D. Benham, Martyn L. Evans, Manfred Caeser |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Patch-Clamp Techniques
medicine.medical_treatment Hippocampal formation Pharmacology Lamotrigine Hippocampus Sodium Channels Membrane Potentials medicine Animals Benzopyrans Patch clamp Cells Cultured Neurons Membrane potential Triazines Chemistry General Neuroscience Sodium channel Embryo Mammalian Rats Carbamazepine medicine.anatomical_structure Anticonvulsant Mechanism of action Phenytoin Benzamides Anticonvulsants Neuron medicine.symptom Sodium Channel Blockers medicine.drug |
| Zdroj: | Neuroscience Letters. 271:57-60 |
| ISSN: | 0304-3940 |
| Popis: | The novel anticonvulsant SB-204269 inhibits epileptiform afterdischarges induced by high K+ in rat hippocampal slices. Its effects on voltage-gated Na+ currents, measured from cultured hippocampal neurones using whole cell patch clamp, were compared to the effects of existing anticonvulsants. SB-204269 produced no significant tonic block of Na+ currents nor any voltage-dependent and frequency-dependent block at doses 50 to 500 fold higher than its anticonvulsant EC50 of 0.2 microM. In contrast, lamotrigine, phenytoin and carbamazepine at 50 microM, blocked Na+ currents in a voltage-dependent manner. SB-204269 also had no effect on action potential discharges evoked by elevating external K+. These data suggest that direct blockade of voltage-gated channels does not contribute to the anticonvulsant properties of SB-204269 and further support the hypothesis that this compound has a novel mechanism of action. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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