High sucrolytic activity by invertase immobilized onto magnetic diatomaceous earth nanoparticles
| Autor: | Caio Rodrigo Dias de Assis, Mariana P. Cabrera, Fernando Soria, David Fernando de Morais Neri, Luiz B. Carvalho, Claudete Fernandes Pereira |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
lcsh:Biotechnology Nanoparticle 01 natural sciences Applied Microbiology and Biotechnology Article Matrix (chemical analysis) Immobilization 010608 biotechnology lcsh:TP248.13-248.65 Residual activity High activity ComputingMethodologies_COMPUTERGRAPHICS chemistry.chemical_classification Chromatography 010405 organic chemistry Chemistry Biomolecule 0104 chemical sciences Invertase Diatomaceous earth Magnetic particle Specific activity Sucrolytic activity Earth (classical element) Biotechnology |
| Zdroj: | Biotechnology Reports, Vol 14, Iss C, Pp 38-46 (2017) Biotechnology Reports |
| Popis: | Graphical abstract Highlights • Innovative biocatalyst with good properties to produce invert sugar. • mDE-APTES-invertase showed 92.5% of residual specific activity. • High sucrolytic activity (3358 U mg−1 protein) by mDE-APTES-invertase was obtained. • Remarkable results of thermal and storage stability, and reuse for mDE-APTES-invertase were found. Invertase immobilized on magnetic diatomaceous earth nanoparticles (mDE-APTES-invertase) with high sucrolytic activity was obtained by an easy and low-cost method. An experimental design was carried out to investigate the best immobilization conditions and it allowed obtaining an immobilized derivative with a residual specific activity equal to 92.5%. Then, a second experimental design selected the mDE-APTES-invertase with higher specific activity in relation to other derivatives reported in the literature (2.42-fold). Thermal and storage stability for immobilized invertase were found to be 35 °C for 60 min (85% retained activity) and 120 days storage period (80% retained activity), respectively. Besides, a residual activity higher than 60% and 50% were observed for mDE-APTES-invertase after reuse in short and long term, respectively. Given the simple and efficient method to obtain an immobilized derivative with high activity, the mDE nanoparticles appear to be a promising matrix for invertase immobilization as well as for other biomolecules. |
| Databáze: | OpenAIRE |
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