Microwave ablation zones are larger than they macroscopically appear - Reevaluation based on NADH vitality staining ex vivo

Autor:	Stefan M Niehues, Christina A Neizert, Kai S. Lehmann, Franz Poch, Janis L Vahldiek, Ole Gemeinhardt, Beatrice Geyer, Robert Klopfleisch
Rok vydání:	2019
Předmět:	Ablation Techniques Pathology medicine.medical_specialty Physiology Swine medicine.medical_treatment 030204 cardiovascular system & hematology 030218 nuclear medicine & medical imaging Lesion 03 medical and health sciences 0302 clinical medicine Physiology (medical) medicine Animals Viability assay Staining and Labeling Chemistry Microwave ablation Histology Hematology Ablation NAD Staining Disease Models Animal Research Design Catheter Ablation medicine.symptom Cardiology and Cardiovascular Medicine Ex vivo Ablation zone
Zdroj:	Clinical hemorheology and microcirculation. 73(2)
ISSN:	1875-8622
Popis:	BACKGROUND Animal liver is established as an ex vivo model for studies on hepatic microwave ablation (MWA). Macroscopically visible color changes in the ablation zone are used to assess cell destruction and confirm successful ablation ex vivo. OBJECTIVE Macroscopy and histology of MWA zones regarding cell viability in ex vivo porcine livers were compared in this study. METHODS Six MWA were performed in porcine livers post mortem. A 14-G antenna and microwave generator (928 MHz; 9.0 kJ) were used. MWA were cut at the maximum cross section in vertical alignment to the antenna. NADH-diaphorase staining determined cell vitality. Macroscopic and microscopic ablation zones were statistically analyzed. RESULTS Histology showed two distinct ablation zones: central white zone (WZH) with no cell viability and peripheral red zone (RZH) with partial cell viability. However, the macroscopically visible WZM was significantly smaller than the microscopic WZH with an area difference of 43.1% (p
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a057cb3c41ebc9ee6883eb6462760bf5 https://pubmed.ncbi.nlm.nih.gov/31156148 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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