Cardiomyocyte Regeneration from Circulating Bone Marrow Cells in Mice
| Autor: | Yohko Uchikoba, Daichi Fukumi, Takashi Shimada, Jun Hayakawa, Yukio Kuramochi, Ryuji Fukazawa, Shunichi Ogawa, Makoto Migita, Mari Hayashida |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Genetically modified mouse
Pathology medicine.medical_specialty Green Fluorescent Proteins Myocardial Infarction Bone Marrow Cells Mice Transgenic Mice Animals Regeneration Medicine Myocyte Myocytes Cardiac Myocardial infarction Bone Marrow Transplantation biology business.industry Myocardium Regeneration (biology) medicine.disease Troponin Mice Inbred C57BL Luminescent Proteins medicine.anatomical_structure Echocardiography Pediatrics Perinatology and Child Health Immunology Circulatory system biology.protein Desmin Bone marrow business |
| Zdroj: | Pediatric Research. 54:319-325 |
| ISSN: | 1530-0447 0031-3998 |
| Popis: | We investigated the role of circulating bone marrow cells (BMC) in cardiomyocyte regeneration. BMC, isolated from transgenic mice expressing enhanced green fluorescent protein (GFP), were transplanted into lethally irradiated C57BL6 mice. Five weeks after bone marrow transplantation (BMT), flow cytometric analysis for GFP-positive cells confirmed reconstitution of transplanted bone marrow. Bone marrow transplant mice subsequently underwent left coronary artery ligation (myocardial infarction) or sham-operation, and were killed at 1 mo or 3 mo after operation. Infarct size was similar in bone marrow transplant mice at 1 mo (47.1 +/- 5.9%) and at 3 mo (45.3 +/- 7.8%), and echocardiography at 2 and 8 wk revealed decreasing left ventricular function. In infarcted heart, GFP-positive cells that expressed desmin and troponin T-C were identified by confocal microscopy. GFP and troponin T-C double-positive cells were predominantly in the peri-infarcted region (1 mo, 365 +/- 45 cells/50 sections; 3 mo: 458 +/- 100 cells/50 sections; p0.05 versus noninfarct, infarct, and sham-operated regions). Furthermore, BMC mobilization and differentiation into cardiomyocytes was found to be complete within 1 mo after myocardial infarction. These results demonstrate that circulating BMC undergo mobilization and differentiation in cardiac cells after myocardial infarction. Future studies are required to determine the molecular signaling mechanisms responsible for this phenomenon. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|