Beetle (Ulomoides dermestoides) fat improves diabetes: effect on liver and pancreatic architecture and on PPARγ expression
| Autor: | L A Prado-Barragan, Rubén Román-Ramos, E.I. Jasso-Villagomez, Julio César Almanza-Pérez, Mario García-Lorenzana, Francisco Javier Alarcón-Aguilar, María de los Angeles Fortis-Barrera, Gerardo Blancas-Flores |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Hypoglycemic agent Male PPARγ Physiology Fat Body H&E stain Peroxisome proliferator-activated receptor Insulin-sensitizing agent Biochemistry 0302 clinical medicine Ulomoides dermestoides General Pharmacology Toxicology and Pharmaceutics lcsh:QH301-705.5 Research Articles chemistry.chemical_classification lcsh:R5-920 geography.geographical_feature_category Glucose Transporter Type 4 biology Chemistry General Neuroscience Diabetes General Medicine Islet Coleoptera medicine.anatomical_structure Liver 030220 oncology & carcinogenesis lcsh:Medicine (General) Pancreas medicine.medical_specialty Immunology Biophysics Ocean Engineering Gas Chromatography-Mass Spectrometry Diabetes Mellitus Experimental 03 medical and health sciences Internal medicine Diabetes mellitus medicine Animals Hypoglycemic Agents RNA Messenger Fatty acids geography Messenger RNA Tissue Extracts Cell Biology medicine.disease PPAR gamma 030104 developmental biology Endocrinology lcsh:Biology (General) Gene Expression Regulation biology.protein GLUT4 |
| Zdroj: | Brazilian Journal of Medical and Biological Research Brazilian Journal of Medical and Biological Research, Volume: 51, Issue: 6, Article number: e7238, Published: 06 APR 2018 Brazilian Journal of Medical and Biological Research, Vol 51, Iss 6 (2018) Brazilian Journal of Medical and Biological Research v.51 n.6 2018 Associação Brasileira de Divulgação Científica (ABDC) instacron:ABDC |
| ISSN: | 1414-431X 0100-879X |
| Popis: | Ulomoides dermestoides is a beetle traditionally consumed to treat diabetes. In this study, we performed a composition analysis of U. dermestoides to obtain the principal fractions, which were used to assess the effect on glycemia, liver and pancreatic architecture, and PPARγ and GLUT4 expression. Normal mice and alloxan-induced diabetic mice were administered fractions of chitin, protein or fat, and the acute hypoglycemic effect was evaluated. A subacute study involving daily administration of these fractions to diabetic mice was also performed over 30 days, after which the liver and pancreas were processed by conventional histological techniques and stained with hematoxylin and eosin to evaluate morphological changes. The most active fraction, the fat fraction, was analyzed by gas chromatography-mass spectrometry (GC-MS), and PPARγ and GLUT4 mRNA expressions were determined in 3T3-L1 adipocytes. The protein and fat fractions exhibited hypoglycemic effects in the acute as well as in the 30-day study. Only the fat fraction led to elevated insulin levels and reduced glycemia, as well as lower intake of water and food. In the liver, we observed recovery of close hepatic cords in the central lobule vein following treatment with the fat fraction, while in the pancreas there was an increased density and percentage of islets and number of cells per islet, suggesting cellular regeneration. The GC-MS analysis of fat revealed three fatty acids as the major components. Finally, increased expression of PPARγ and GLUT4 was observed in 3T3-L1 adipocytes, indicating an antidiabetic effect. |
| Databáze: | OpenAIRE |
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