Beetle (Ulomoides dermestoides) fat improves diabetes: effect on liver and pancreatic architecture and on PPARγ expression

Autor: L A Prado-Barragan, Rubén Román-Ramos, E.I. Jasso-Villagomez, Julio César Almanza-Pérez, Mario García-Lorenzana, Francisco Javier Alarcón-Aguilar, María de los Angeles Fortis-Barrera, Gerardo Blancas-Flores
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
Hypoglycemic agent
Male
PPARγ
Physiology
Fat Body
H&E stain
Peroxisome proliferator-activated receptor
Insulin-sensitizing agent
Biochemistry
0302 clinical medicine
Ulomoides dermestoides
General Pharmacology
Toxicology and Pharmaceutics
lcsh:QH301-705.5
Research Articles
chemistry.chemical_classification
lcsh:R5-920
geography.geographical_feature_category
Glucose Transporter Type 4
biology
Chemistry
General Neuroscience
Diabetes
General Medicine
Islet
Coleoptera
medicine.anatomical_structure
Liver
030220 oncology & carcinogenesis
lcsh:Medicine (General)
Pancreas
medicine.medical_specialty
Immunology
Biophysics
Ocean Engineering
Gas Chromatography-Mass Spectrometry
Diabetes Mellitus
Experimental
03 medical and health sciences
Internal medicine
Diabetes mellitus
medicine
Animals
Hypoglycemic Agents
RNA
Messenger
Fatty acids
geography
Messenger RNA
Tissue Extracts
Cell Biology
medicine.disease
PPAR gamma
030104 developmental biology
Endocrinology
lcsh:Biology (General)
Gene Expression Regulation
biology.protein
GLUT4
Zdroj: Brazilian Journal of Medical and Biological Research
Brazilian Journal of Medical and Biological Research, Volume: 51, Issue: 6, Article number: e7238, Published: 06 APR 2018
Brazilian Journal of Medical and Biological Research, Vol 51, Iss 6 (2018)
Brazilian Journal of Medical and Biological Research v.51 n.6 2018
Associação Brasileira de Divulgação Científica (ABDC)
instacron:ABDC
ISSN: 1414-431X
0100-879X
Popis: Ulomoides dermestoides is a beetle traditionally consumed to treat diabetes. In this study, we performed a composition analysis of U. dermestoides to obtain the principal fractions, which were used to assess the effect on glycemia, liver and pancreatic architecture, and PPARγ and GLUT4 expression. Normal mice and alloxan-induced diabetic mice were administered fractions of chitin, protein or fat, and the acute hypoglycemic effect was evaluated. A subacute study involving daily administration of these fractions to diabetic mice was also performed over 30 days, after which the liver and pancreas were processed by conventional histological techniques and stained with hematoxylin and eosin to evaluate morphological changes. The most active fraction, the fat fraction, was analyzed by gas chromatography-mass spectrometry (GC-MS), and PPARγ and GLUT4 mRNA expressions were determined in 3T3-L1 adipocytes. The protein and fat fractions exhibited hypoglycemic effects in the acute as well as in the 30-day study. Only the fat fraction led to elevated insulin levels and reduced glycemia, as well as lower intake of water and food. In the liver, we observed recovery of close hepatic cords in the central lobule vein following treatment with the fat fraction, while in the pancreas there was an increased density and percentage of islets and number of cells per islet, suggesting cellular regeneration. The GC-MS analysis of fat revealed three fatty acids as the major components. Finally, increased expression of PPARγ and GLUT4 was observed in 3T3-L1 adipocytes, indicating an antidiabetic effect.
Databáze: OpenAIRE
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