Imaging and Detection of Carboxylesterase in Living Cells and Zebrafish Pretreated with Pesticides by a New Near-Infrared Fluorescence Off-On Probe
| Autor: | Weihua Chen, Dongyu Li, Zhao Li, Xingbin Yang |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Fluorescence-lifetime imaging microscopy
Fluorophore Analytical chemistry 010402 general chemistry Photochemistry 01 natural sciences Carboxylesterase Cell membrane chemistry.chemical_compound medicine Moiety Animals Humans Enzyme Inhibitors Pesticides Zebrafish Fluorescent Dyes Detection limit Quenching (fluorescence) 010405 organic chemistry Chemistry General Chemistry Zebrafish Proteins Fluorescence 0104 chemical sciences medicine.anatomical_structure Spectrometry Fluorescence General Agricultural and Biological Sciences HeLa Cells |
| Zdroj: | Journal of agricultural and food chemistry. 65(20) |
| ISSN: | 1520-5118 |
| Popis: | A new near-infrared fluorescence off–on probe was developed and applied to fluorescence imaging of carboxylesterase in living HepG-2 cells and zebrafish pretreated with pesticides (carbamate, organophosphorus, and pyrethroid). The probe was readily prepared by connecting (4-acetoxybenzyl)oxy as a quenching and recognizing moiety to a stable hemicyanine skeleton that can be formed via the decomposition of IR-780. The fluorescence off–on response of the probe to carboxylesterase is based on the enzyme-catalyzed spontaneous hydrolysis of the carboxylic ester bond, followed by a further fragmentation of the phenylmethyl unit and thereby the fluorophore release. Compared with the only existing near-infrared carboxylesterase probe, the proposed probe exhibits superior analytical performance, such as near-infrared fluorescence emission over 700 nm as well as high selectivity and sensitivity, with a detection limit of 4.5 × 10–3 U/mL. More importantly, the probe is cell membrane permeable, and its applicability h... |
| Databáze: | OpenAIRE |
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