Evaluation of in vitro antioxidant and apoptotic activities of Cyperus rotundus

Autor: Ghedira Zied, Ghedira Chekir Leila, Krifa Mounira, Kilani-Jaziri Soumaya, Nasr Nouha, Ghedira Kamel, Franca Dijoux Marie Genviève
Přispěvatelé: Ecologie microbienne ( EM ), Centre National de la Recherche Scientifique ( CNRS ) -Ecole Nationale Vétérinaire de Lyon ( ENVL ) -Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique ( INRA ) -VetAgro Sup ( VAS ), Laboratoire d'Ecologie Microbienne - UMR 5557 (LEM), Centre National de la Recherche Scientifique (CNRS)-Ecole Nationale Vétérinaire de Lyon (ENVL)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Ecole Nationale Vétérinaire de Lyon (ENVL)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2014
Předmět:
[SDV.SA]Life Sciences [q-bio]/Agricultural sciences
[ SDV.BV ] Life Sciences [q-bio]/Vegetal Biology
Antioxidant
medicine.medical_treatment
[ SDV.TOX ] Life Sciences [q-bio]/Toxicology
Apoptosis
DNA fragmentation
Deoxyribose assay
Antioxidants
Lipid peroxidation
chemistry.chemical_compound
0302 clinical medicine
Glucosides
Cyperus
[ SDV.SA ] Life Sciences [q-bio]/Agricultural sciences
Orientin
Medicine(all)
[SDV.EE]Life Sciences [q-bio]/Ecology
environment
0303 health sciences
General Medicine
Biochemistry
030220 oncology & carcinogenesis
[SDV.TOX]Life Sciences [q-bio]/Toxicology
Xanthine/Xanthine oxidase assay
Xanthine Oxidase
[SDV.BID]Life Sciences [q-bio]/Biodiversity
[ SDV.EE ] Life Sciences [q-bio]/Ecology
environment
03 medical and health sciences
medicine
Humans
[SDV.BV]Life Sciences [q-bio]/Vegetal Biology
MTT assay
[SDV.BBM]Life Sciences [q-bio]/Biochemistry
Molecular Biology
Xanthine oxidase
[ SDV.BBM ] Life Sciences [q-bio]/Biochemistry
Molecular Biology
030304 developmental biology
Cell Proliferation
Flavonoids
[ SDV.BID ] Life Sciences [q-bio]/Biodiversity
Chromatography
Plant Extracts
Polyphenols
Xanthine
chemistry
Polyphenol
Lipid Peroxidation
K562 Cells
Lipid peroxidation assay
Cyperus rotundus
Zdroj: Asian Pacific Journal of Tropical Medicine
Asian Pacific Journal of Tropical Medicine, 2014, 7 (2), pp.105-112. 〈10.1016/S1995-7645(14)60004-3〉
asian pacific journal of tropical medecine
asian pacific journal of tropical medecine, 2014, 7 (2), pp.105-112. ⟨10.1016/S1995-7645(14)60004-3⟩
asian pacific journal of tropical medecine, 2014, 7 (2), pp.105-112. 〈10.1016/S1995-7645(14)60004-3〉
DOI: 10.1016/S1995-7645(14)60004-3〉
Popis: Objective To evaluate in vitro antioxidant and apoptotic activities of Cyperus rotundus ( C. rotundus ). Methods The phytochemical study and the antioxidant activities of both methanol and aqueous extracts from C. rotundus aerial part were determined. In addition, these extracts were also investigated for their cytotoxic and apoptotic activities. The major compound of the methanol extract was isolated. Both methanol and aqueous extracts (300, 150, and 50 μg/mL) were evaluated for their antioxidant activity by the xanthine/xanthine oxidase assay system. However, 16, 8, and 4 mg/mL of each extract were tested to investigate their OH. formation scavenging potential. Aqueous extract (800, 400, and 200 μg/mL) and methanol extract (350, 175, and 88 μg/mL) were tested against lipid peroxidation, induced by 75 μM H 2 O 2 . The cytotoxicity (by MTT assay) and cell DNA fragmentation of both extracts were evaluated towards K562 and L1210 cell lines. The major compound was obtained from the butanol fraction of methanol extract and its structure was determined by RMN spectroscopic analysis. Results The methanol and aqueous extracts showed respectively, 88% and 19% inhibition of xanthine oxidase activity. Yet, the same extracts inhibited lipid peroxidation by 61.5% and 42.0%, respectively. Both extracts inhibited OH. formation by 27.1% and 25.3%, respectively. Only methanol extract induced DNA degradation. Orientin was determined as the major compound isolated from the butanol fraction of methanol extract. Conclusions It appears that C. rotundus extracts exhibit a potential use as a natural antioxidant and an apoptosis inducer.
Databáze: OpenAIRE
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