The role of phenylalanine hydroxylase in melanotic encapsulation of filarial worms in two species of mosquitoes
| Autor: | Jeremy F. Fuchs, Stephen Higgs, Jody K. Johnson, Thomas A. Rocheleau, Bruce M. Christensen, Lyric C. Bartholomay, Cheng-Chen Chen, Li-Chun Infanger |
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| Rok vydání: | 2004 |
| Předmět: |
Transcription
Genetic Phenylalanine hydroxylase Dirofilaria immitis Molecular Sequence Data Phenylalanine Aedes aegypti Biology Biochemistry Microbiology Melanin Aedes parasitic diseases Botany Animals Amino Acid Sequence Tyrosine Molecular Biology RNA Double-Stranded Melanins chemistry.chemical_classification Gene knockdown Reverse Transcriptase Polymerase Chain Reaction fungi Phenylalanine Hydroxylase biology.organism_classification Immunity Innate Recombinant Proteins RNA silencing Phenotype Enzyme chemistry Insect Science biology.protein Female |
| Zdroj: | Insect Biochemistry and Molecular Biology. 34:1329-1338 |
| ISSN: | 0965-1748 |
| Popis: | Melanin formation has a significant influence on mosquito vector competence by limiting the development of metazoan parasites. Tyrosine, the rate-limiting substrate of melanin production, can be obtained exogenously or derived from phenylalanine by phenylalanine hydroxylase (PAH). The characteristics of this defense mechanism, such as temporal expression of constituent enzymes involved in the biosynthetic pathway, can vary considerably between mosquito species. We investigated the functional role of PAH in the melanotic encapsulation response in Aedes aegypti and Armigeres subalbatus, two mosquito species with markedly different melanization responses. We used double-stranded RNA (dsRNA) to knock down PAH and observed the phenotypic effects on melanin formation. PAH transcripts were dramatically reduced in both mosquito species after gene knock down. The abundance of PAH proteins was decreased in gene knockdown mosquitoes that were inoculated with Dirofilaria immitis microfilariae (mf) as compared to inoculation controls. A significant reduction of mf melanization also was observed in these knockdown mosquitoes as compared to inoculation controls. Our data suggest that PAH is required for a fully functional melanotic encapsulation response in both mosquito vectors. |
| Databáze: | OpenAIRE |
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