Decreased DNA-PK activity in human cancer cells exhibiting hypersensitivity to low-dose irradiation
| Autor: | Eric Deutsch, François Eschwege, Philippe Lambin, Michael C. Joiner, S Vaganay-Juéry, Jean Bourhis, Patrick Calsou, Bassam Abdulkarim, Catherine Muller, Bernard Salles, Elisabetta Marangoni |
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| Rok vydání: | 2000 |
| Předmět: |
Cancer Research
DNA Repair DNA repair Blotting Western Molecular Sequence Data DNA-Activated Protein Kinase Protein Serine-Threonine Kinases Biology Radiation Tolerance Ionizing radiation DNA-PK chemistry.chemical_compound Tumor Cells Cultured Humans Amino Acid Sequence Radiosensitivity Kinase activity Protein kinase A Nuclear Proteins Dose-Response Relationship Radiation Regular Article low-dose hyper-radiosensitivity Molecular biology In vitro DNA-Binding Proteins Oncology Biochemistry chemistry Cell culture ionizing radiation DNA |
| Zdroj: | British Journal of Cancer |
| ISSN: | 1532-1827 0007-0920 |
| DOI: | 10.1054/bjoc.2000.1258 |
| Popis: | Low-dose hyper-radiosensitivity (HRS) (below 0.5 Gy) has been extensively documented in the past few years. The molecular basis of this phenomenon remains largely unknown and the purpose of this study was to investigate the possible implication of the DNA repair DNA-PK complex. The activity of the DNA-PK complex, i.e. Ku DNA-end binding activity and kinase activity of the whole complex, was studied in 10 human cancer cell lines, 2 h after 0.2, 0.5 and 1 Gy irradiation. After low-dose irradiation (0.2 Gy), a marked decrease in DNA-PK activity was found in all six cell lines exhibiting HRS, whereas the DNA-PK activity was increased in the four cell lines which did not exhibit HRS. This modulation of DNA-PK activity was a rapid phenomenon occurring within the 2 h following low-dose radiation exposure. These data strongly suggest the implication of the DNA-PK repair complex in the HRS phenomenon. © 2000 Cancer Research Campaign |
| Databáze: | OpenAIRE |
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