A critical assessment of transmethylation procedures for n-3 long-chain polyunsaturated fatty acid quantification of lipid classes
| Autor: | Laurence Fonseca, Leslie Couëdelo, Anthony Sehl, Carole Vaysse, Maud Cansell |
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| Přispěvatelé: | Chimie et Biologie des Membranes et des Nanoobjets (CBMN), École Nationale d'Ingénieurs des Travaux Agricoles - Bordeaux (ENITAB)-Institut de Chimie du CNRS (INC)-Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS), ITERG, Université Sciences et Technologies - Bordeaux 1-ITERG |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Chromatography Gas Food chemistry Fatty acid methyl esters Sodium methoxide Methylation Analytical Chemistry 03 medical and health sciences chemistry.chemical_compound Fatty Acids Omega-3 Animals Humans High performance thin layer chromatography Phospholipids Triglycerides chemistry.chemical_classification Gas chromatography 030109 nutrition & dietetics Chromatography Chemistry Methanol High-performance thin layer chromatography [CHIM.MATE]Chemical Sciences/Material chemistry General Medicine Sulfuric Acids Lipids Sterol Rats 030104 developmental biology Fatty Acids Unsaturated Polyunsaturated fatty acids lipids (amino acids peptides and proteins) Quantitative analysis (chemistry) Transmethylation Food Science Polyunsaturated fatty acid |
| Zdroj: | Food Chemistry Food Chemistry, Elsevier, In press, 251, pp.1-8. ⟨10.1016/j.foodchem.2018.01.060⟩ |
| ISSN: | 1873-7072 0308-8146 |
| DOI: | 10.1016/j.foodchem.2018.01.060⟩ |
| Popis: | Lipid transmethylation methods described in the literature are not always evaluated with care so to insure that the methods are effective, especially on food matrix or biological samples containing polyunsaturated fatty acid (PUFA). The aim of the present study was to select a method suitable for all lipid species rich in long chain n-3 PUFA. Three published methods were adapted and applied on individual lipid classes. Lipid (trans)methylation efficiency was characterized in terms of reaction yield and gas chromatography (GC) analysis. The acid-catalyzed method was unable to convert triglycerides and sterol esters, while the method using an incubation at a moderate temperature was ineffective on phospholipids and sterol esters. On the whole only the method using sodium methoxide and sulfuric acid was effective on lipid classes taken individually or in a complex medium. This study highlighted the use of an appropriate (trans)methylation method for insuring an accurate fatty acid composition. |
| Databáze: | OpenAIRE |
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