Hyperoxia induces glutamine-fuelled anaplerosis in retinal Müller cells
| Autor: | Youstina Bolok, Vincent Tran, Alex Yuan, Leah McCollum, Henri Brunengraber, George Hoppe, Jonathan E. Sears, Charandeep Singh, Kristin Allan |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
genetic structures Glutamine General Physics and Astronomy Mice 0302 clinical medicine Glycolysis Phosphorylation lcsh:Science Cells Cultured Hyperoxia Carbon Isotopes Multidisciplinary Molecular medicine Chemistry respiratory system 3. Good health Cell biology Mitochondria medicine.anatomical_structure Metabolome medicine.symptom Muller glia Oxidation-Reduction Science Ependymoglial Cells Pyruvate Dehydrogenase Complex Models Biological General Biochemistry Genetics and Molecular Biology Article 03 medical and health sciences Glutaminase medicine Animals Humans Metabolomics Retina Catabolism Endothelial Cells General Chemistry Metabolism eye diseases Citric acid cycle 030104 developmental biology Glucose Astrocytes lcsh:Q sense organs 030217 neurology & neurosurgery |
| Zdroj: | Nature Communications Nature Communications, Vol 11, Iss 1, Pp 1-11 (2020) |
| ISSN: | 2041-1723 |
| Popis: | Although supplemental oxygen is required to promote survival of severely premature infants, hyperoxia is simultaneously harmful to premature developing tissues such as in the retina. Here we report the effect of hyperoxia on central carbon metabolism in primary mouse Müller glial cells and a human Müller glia cell line (M10-M1 cells). We found decreased flux from glycolysis entering the tricarboxylic acid cycle in Müller cells accompanied by increased glutamine consumption in response to hyperoxia. In hyperoxia, anaplerotic catabolism of glutamine by Müller cells increased ammonium release two-fold. Hyperoxia induces glutamine-fueled anaplerosis that reverses basal Müller cell metabolism from production to consumption of glutamine. Prematurely born babies need extra oxygen to survive, but this can cause damage to the eyes and lead to infant blindness. Here the authors show that this hyperoxia changes the metabolism of Müller cells in the retina such that they use up, rather than produce, glutamine and secrete excess ammonium. |
| Databáze: | OpenAIRE |
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