In Vitro Unfolding of Insulin: Characterization of Intermediates and Putative Unfolding Pathway
| Autor: | You-Min Feng, Yue-Hua Tang, Jie Zhao, Fu-Kun Zhao, Qi-Long Huang |
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| Rok vydání: | 2010 |
| Předmět: |
Models
Molecular Protein Folding Spectrometry Mass Electrospray Ionization Swine medicine.medical_treatment Molecular Sequence Data Equilibrium unfolding Biochemistry Structural Biology medicine Animals Humans Insulin Amino Acid Sequence Disulfides Chromatography High Pressure Liquid Chemistry Circular Dichroism Disulfide bond General Medicine In vitro Folding (chemistry) Oxidized Glutathione biological sciences Biophysics Electrophoresis Polyacrylamide Gel |
| Zdroj: | Protein & Peptide Letters. 17:874-880 |
| ISSN: | 0929-8665 |
| DOI: | 10.2174/092986610791306724 |
| Popis: | The in vitro insulin unfolding had been studied using the "equilibrium unfolding" method where protein is unfolded by reducing reagents in the presence of trace amounts of oxidants such as oxidized glutathione. Nine intermediates were captured in the unfolding process, named as P1A, P2A, P3A, P4A, P3B, P4B, P5B, P6B, and P7B, which were all either A chain derivatives or B chain derivatives. No intermediate with inter-A-B chain disulfide was captured. Based on the character of the intermediates, their distribution during the unfolding process and the hypothetic "transient" intermediates, an in vitro putative unfolding pathway of insulin had been proposed. Besides, the comparison of the intermediates captured in unfolding with the intermediates captured in the refolding process of insulin revealed that both unfolding/refolding processes of insulin shared common intermediates. Based on these observations we suggested that the unfolding pathway of insulin was similar to the refolding pathway but flowed in the opposite direction. |
| Databáze: | OpenAIRE |
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