Silencing JA hydroxylases in Nicotiana attenuata enhances jasmonic acid-isoleucine-mediated defenses against Spodoptera litura
| Autor: | Lei Wang, Suiyun Chen, Jianqiang Wu, Tang Jinxiang, Dahai Yang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Types of tobacco Oxygenase Herbivore defense Spodoptera litura Plant Science Biology 010603 evolutionary biology 01 natural sciences Article 12-OH-JA Hydroxylation chemistry.chemical_compound Nicotiana attenuata lcsh:Botany Plant defense against herbivory Gene silencing lcsh:QH301-705.5 Ecology Evolution Behavior and Systematics Jasmonic acid fungi 2-Oxoglutarate oxygenase food and beverages JA metabolism biology.organism_classification Cell biology lcsh:QK1-989 chemistry lcsh:Biology (General) 010606 plant biology & botany |
| Zdroj: | Plant Diversity, Vol 42, Iss 2, Pp 111-119 (2020) Plant Diversity |
| ISSN: | 2468-2659 |
| Popis: | Jasmonic acid (JA) plays important roles in plant resistance to insect herbivores. One important derivative of JA is 12-OH-JA, which is produced by two independent pathways: direct hydroxylation of JA by jasmonate-induced oxygenases (JOXs) or hydrolyzation of 12-OH-JA-Ile.Yet the function of 12-OH-JA in plant–herbivore interactions remains largely unknown. In this study, we silenced four JOX homologs independently in the wild tobacco Nicotiana attenuata by virus-induced gene silencing (VIGS), and found that all four JOX homologs are involved in JA hydroxylation. Simultaneously silencing the four JA hydroxylases in VIGS-NaJOXs plants decreased herbivory-induced 12-OH-JA by 33%, but JA and JA-Ile levels increased by 45% and 30%, respectively, compared to those in control plants. Compared to direct hydroxylation from JA, hydrolyzation from 12-OH-JA-Ile is equally important for herbivory-induced 12-OH-JA accumulation: in the 12-OH-JA-Ile deficient irJAR4/6 plants, 12-OH-JA decreased 34%. Moreover, VIGS-NaJOXs plants exhibited enhanced resistance to the generalist herbivore Spodoptera litura. The poor larval performance was strongly correlated with high levels of several JA-Ile-dependent direct defense metabolites in the VIGS-NaJOXs plants. When we simultaneously silenced all four JA hydroxylases in the JA-Ile-deficient irJAR4/6 background, the enhanced herbivore resistance diminished, demonstrating that enhanced herbivore resistance resulted from elevated JA-Ile levels. Given that silencing these NaJOX-like genes did not detectably alter plant growth but highly increased plant defense levels, we propose that JOX genes are potential targets for genetic improvement of herbivore-resistant crops. Keywords: JA metabolism, 12-OH-JA, Spodoptera litura, 2-Oxoglutarate oxygenase, Herbivore defense, Nicotiana attenuata |
| Databáze: | OpenAIRE |
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