Relationship of Streptococcus mutans with valvar cardiac tissue: A molecular and immunohistochemical study
| Autor: | Paulo Goberlânio de Barros Silva, Fabrício Bitu Sousa, Mário Rogério Lima Mota, Clarissa Pessoa Fernandes Forte, Ândrea Kely Campos Ribeiro dos Santos, Ana Paula Negreiros Nunes Alves, Francisco Artur Forte Oliveira, Camile de Barros Lopes, Raquel Carvalho Montenegro |
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| Rok vydání: | 2019 |
| Předmět: |
Cancer Research
Pathology medicine.medical_specialty Dental Plaque Dental plaque Pathology and Forensic Medicine Streptococcus mutans 03 medical and health sciences 0302 clinical medicine medicine.artery medicine Humans Aged Aorta biology business.industry Calcinosis Aortic Valve Stenosis 030206 dentistry Middle Aged medicine.disease biology.organism_classification Immunohistochemistry Molecular analysis Stenosis Real-time polymerase chain reaction Otorhinolaryngology Aortic Valve 030220 oncology & carcinogenesis cardiovascular system Periodontics Oral Surgery business CD61 |
| Zdroj: | Journal of Oral Pathology & Medicine. 48:745-753 |
| ISSN: | 1600-0714 0904-2512 |
| DOI: | 10.1111/jop.12929 |
| Popis: | Background The present study aimed to investigate the presence or absence of Streptococcus mutans in oral cavity and valvular samples associating with the histomorphologic alterations of calcified aortic stenosis. Methodology Dental plaque and cardiac valve samples were collected from 10 patients with calcified aortic stenosis for molecular analysis of S mutans by real-time polymerase chain reaction (PCR). Healthy valve tissue was also collected from five young cadavers and analyzed for S mutans. Moreover, fragments of all valvar specimens were submitted for histomorphological analysis and immunohistochemistry (anti-S mutans and anti-CD61). Results Streptococcus mutans was present in 100% of the oral cavity samples from the patients with calcified aortic stenosis in the molecular analysis. The analysis by real-time PCR showed that S mutans presented the same proportion in healthy valves and those with calcified aortic stenosis (80%; P = 1.000). Conversely, the immunoexpression of S mutans was 37.40 (IC95% = 1.49-937.00) times superior in samples of patients with cardiac disease (P = .007). The immunoexpression analysis showed that CD61 was present in seven (70%) calcified aortic stenosis samples, all of which were also immunopositive for S mutans. Conclusions Streptococcus mutans was found in the oral cavity, healthy valve tissue, and calcified aortic stenosis samples. However, the microorganism was visualized by immunohistochemistry only in the calcified aortic stenosis samples, which may suggest viability and an increased bacterial density in this condition. The association of the presence of S mutans and positive CD61 immunoexpression suggests a probable relationship with calcified aortic stenosis. |
| Databáze: | OpenAIRE |
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