Establishment of an in Vitro Human Blood-Brain Barrier Model Derived from Induced Pluripotent Stem Cells and Comparison to a Porcine Cell-Based System
| Autor: | Ivan Fini, Maria Veneziano, Vinod Khetarpal, Elena Bracacel, Todd Herbst, Ignacio Munoz-Sanjuan, Mark Rose, Giulio Auciello, Edith Monteagudo, Alessandro Rosa, Celia Dominguez, Odalys Gonzalez Paz, Antonella Cellucci, Annalise Di Marco, Maria Rosaria Battista, Isabelle Gloaguen, Domenico Vignone |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Central Nervous System
Swine Induced Pluripotent Stem Cells Central nervous system blood brain barrier Blood–brain barrier human induced pluripotent stem cells CNS permeability Huntington’s disease Article medicine Animals Humans Induced pluripotent stem cell Receptor lcsh:QH301-705.5 Cells Cultured Cryopreservation Chemistry Brain Endothelial Cells Biological Transport Cell Differentiation General Medicine Immunohistochemistry In vitro Cell biology medicine.anatomical_structure lcsh:Biology (General) Blood-Brain Barrier Astrocytes Paracellular transport Stem cell Homeostasis |
| Zdroj: | Cells Volume 9 Issue 4 Cells, Vol 9, Iss 994, p 994 (2020) |
| Popis: | The blood-brain barrier (BBB) is responsible for the homeostasis between the cerebral vasculature and the brain and it has a key role in regulating the influx and efflux of substances, in healthy and diseased states. Stem cell technology offers the opportunity to use human brain-specific cells to establish in vitro BBB models. Here, we describe the establishment of a human BBB model in a two-dimensional monolayer culture, derived from human induced pluripotent stem cells (hiPSCs). This model was characterized by a transendothelial electrical resistance (TEER) higher than 2000 Ω∙cm2 and associated with negligible paracellular transport. The hiPSC-derived BBB model maintained the functionality of major endothelial transporter proteins and receptors. Some proprietary molecules from our central nervous system (CNS) programs were evaluated revealing comparable permeability in the human model and in the model from primary porcine brain endothelial cells (PBECs). |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|