Activated human Langerhans cells express mRNA for IL-1 alpha and IL-1 beta and produce these cytokines but do not secrete them
| Autor: | Vera B. Morhenn, Mariola Ilnicka, Simon W. Lee, Elsie M. Eugui |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Lipopolysaccharides
Lipopolysaccharide Immunology Antigen presentation Gene Expression Biology In Vitro Techniques Biochemistry chemistry.chemical_compound Immunology and Allergy Humans RNA Messenger Molecular Biology Messenger RNA Ionomycin Interleukin RNA Hematology Molecular biology chemistry Langerhans Cells Tetradecanoylphorbol Acetate Tumor necrosis factor alpha Acetylmuramyl-Alanyl-Isoglutamine Muramyl dipeptide Interleukin-1 |
| Zdroj: | Cytokine. 4(6) |
| ISSN: | 1043-4666 |
| Popis: | Human Langerhans cells (LC) were isolated from epidermal cell preparations by panning with mouse anti-CD1 monoclonal antibody. RNA was prepared and probed for the presence of mRNAs for various cytokines using radiolabeled cDNAs. After stimulation with phorbol myristate acetate LC express RNA for interleukin 1 alpha (IL-1 alpha) and interleukin 1 beta (IL-1 beta) and produce proteins but do not secrete them at detectable levels. LC-associated IL-1, particularly IL-1 alpha, may play a role in antigen presentation. PMA did not induce IL-6 expression in LC. The addition of lipopolysaccharide, a muramyl dipeptide analog, ionomycin, IL-1 alpha, tumor necrosis factor-alpha, insulin-like growth factor-1 or IL-6 did not induce IL-1 mRNA in LC. UVB augmented IL-1 beta mRNA expression. Glucocorticoids did not detectably affect IL-1 alpha or IL-1 beta mRNA levels following PMA induction, however, staurosporin inhibited IL-1 beta mRNA synthesis. Thus the inducers and regulators of IL-1 formation in human LC and monocytes are not identical. |
| Databáze: | OpenAIRE |
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