Identification of sulfurtransferase enzymes in Azotobacter vinelandii
| Autor: | Silvia Pagani, C. Kennedy, E. Franchi, Rita Colnaghi |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Nitrogen
Ion chromatography Biophysics Sulfurtransferase Rhodanese Biochemistry Structural Biology Genetics Molecular Biology Azotobacteraceae Sulphurtransferase chemistry.chemical_classification Azotobacter vinelandii Chromatography biology 3-Mercaptopyruvate sulphurtransferase Fast protein liquid chromatography Cell Biology biology.organism_classification Thiosulfate Sulfurtransferase Molecular Weight Enzyme chemistry Azotobacter Sulfurtransferases Electrophoresis Polyacrylamide Gel Bacteria |
| Zdroj: | FEBS letters. 278(2) |
| ISSN: | 0014-5793 |
| Popis: | Rhodanese and 3-mercaptopyruvate sulphurtransferase have been identified in A. vinelandii . Two distinct active fractions of the two sulphurtransferases were obtained after FPLC ion-exchange chromatography of material partially purified from crude extracts. Rhodanese has been purified to homogeneity, and it consists of one polypeptide chain of M r ca 25 000. A partial purification of 3-mercaptopyruvate sulphurtransferase was obtained. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text