Correlation between the suppressor of cytokine signaling-1 and 3 and hepatitis B virus: possible roles in the resistance to interferon treatment

Autor: Xing Cheng, Yao-Li Cui, En-Qiang Chen, Hong Tang, Li Liu, Lingyao Du
Rok vydání: 2014
Předmět:
Male
Biopsy
medicine.medical_treatment
Drug Resistance
Suppressor of Cytokine Signaling Proteins
medicine.disease_cause
Polyethylene Glycols
Mice
Pegylated interferon
Interferon
SOCS3
Mice
Inbred BALB C
Middle Aged
Viral Load
Hepatitis B
SOCS
Recombinant Proteins
Treatment Outcome
Infectious Diseases
Cytokine
Liver
Female
Viral load
psychological phenomena and processes
medicine.drug
inorganic chemicals
Adult
Hepatitis B virus
Adolescent
Interferon alpha-2
Biology
behavioral disciplines and activities
Young Adult
Hepatitis B
Chronic
Suppressor of Cytokine Signaling 1 Protein
Virology
otorhinolaryngologic diseases
medicine
Animals
Humans
Aged
Suppressor of cytokine signaling 1
Gene Expression Profiling
Research
Interferon-alpha
PEG-IFN α-2b
medicine.disease
Disease Models
Animal
Poly I-C
Suppressor of Cytokine Signaling 3 Protein
Immunology
sense organs
Chronic Hepatitis B
Zdroj: Virology Journal
ISSN: 1743-422X
Popis: Background The suppressor of cytokine signaling family (SOCS) is an important negative regulator in the JAK-STAT signaling pathway. This study was designed to explore the correlation between SOCS-1, 2 and 3, Hepatitis B Virus (HBV) and interferon (IFN), and the relationship between SOCS and IFN therapeutic efficacy. Methods Four types of mouse models were established. Mice were administered with HBV replicative plasmid pHBV4.1 and IFN inducer Poly IC (Group A), pHBV4.1 (Group B), Poly IC (Group C) and saline (Group D), respectively. Liver tissues were harvested from the mice and SOCS expression was determined. Meanwhile, patients with chronic hepatitis B (CHB) were treated with pegylated interferon α-2b for 24-48 weeks. Liver biopsy was collected and the baseline SOCS expression was determined. Serum assay was performed for efficacy evaluation and correlation analysis. Results In animal studies, the expression level of SOCS-1 and 3 was found in the descending order of B, A, C and D. The difference between Group B and D suggested that HBV could induce SOCS. The difference between Group A and C suggested that HBV could still induce SOCS with up-regulated endogenous IFN. The difference between Group C and D suggested that ploy IC could induce SOCS, while the difference between Group B and A suggested that Poly IC might have a stronger inhibition effect for SOCS. There was no difference in SOCS-2 expression. In clinical studies, eight of twenty-four enrolled patients achieved either complete or partial therapeutic response. The expression of both SOCS-1 and 3 was higher in CHB patients than in normal controls. The baseline HBV-DNA level was positively correlated with SOCS-1 and 3. The age, viral genotype, HBVDNA, SOCS-1 and SOCS-3 were found to be related to IFN efficacy. Conclusion HBV could induce both SOCS-1 and 3 expression regardless of endogenous IFN level. Elevated IFN could directly up-regulate SOCS-1 and 3 expression, but it could also indirectly down-regulate SOCS-1 and 3 expression by inhibiting HBV replication. HBV might play a more important role in the SOCS up-regulation than IFN, a possible reason why patients with high HBV viral load encounter poor efficacy of IFN treatment.
Databáze: OpenAIRE
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