Detection of Genomic DNA Fragmentation during Apoptosis (DNA Ladder) and the Simultaneous Isolation of RNA from Low Cell Numbers
| Autor: | Isrid Sturm, Peter Bendzko, Peter T. Daniel, Bernd Dörken, Silke Ritschel, Katrin Friedrich, Timo Hillebrand |
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| Rok vydání: | 1999 |
| Předmět: |
T-Lymphocytes
Biophysics Apoptosis DNA Fragmentation Thymus Gland Biology Sensitivity and Specificity Biochemistry Jurkat Cells Mice chemistry.chemical_compound Gene expression Animals Humans fas Receptor Fragmentation (cell biology) Molecular Biology Cells Cultured Gel electrophoresis Mice Inbred BALB C Reverse Transcriptase Polymerase Chain Reaction Apoptotic DNA fragmentation Antibodies Monoclonal RNA DNA Cell Biology Molecular biology Molecular Weight genomic DNA chemistry DNA fragmentation Female Adsorption |
| Zdroj: | Analytical Biochemistry. 266:110-115 |
| ISSN: | 0003-2697 |
| Popis: | In the present paper we describe a rapid and sensitive method for the simultaneous isolation of total RNA and genomic plus low-molecular-weight DNA from apoptotic cells. Using this method, we were able to detect a DNA ladder from as low as 30,000 apoptotic cells in only 45 min including gel electrophoresis. In addition, RNA can be readily obtained from the same specimen to assess gene expression during apoptosis. This method therefore appears to be advantageous when sensitivity and low amounts of sample material are a limiting factor. |
| Databáze: | OpenAIRE |
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