Synthesis of Macroporous Poly(dimethylsiloxane) Scaffolds for Tissue Engineering Applications
| Autor: | Christopher A. Fraker, Cherie L. Stabler, Ann Christina Brady, Eileen Pedraza |
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| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Scaffold
Materials science Biocompatibility Surface Properties Biomedical Engineering Biophysics Bioengineering Biocompatible Materials Matrix (biology) Article Biomaterials Tissue engineering Materials Testing Animals Humans Dimethylpolysiloxanes Composite material Porosity Cells Cultured biology Tissue Engineering Tissue Scaffolds technology industry and agriculture Mesenchymal Stem Cells Biocompatible material Solvent casting and particulate leaching Rats Fibronectin Chemical engineering Rats Inbred Lew biology.protein |
| Popis: | Macroporous, biostable scaffolds with controlled porous architecture were prepared from poly(dimethylsiloxane) (PDMS) using sodium chloride particles (NaCl) and a solvent casting and particulate leaching (SCPL) technique. The effect of particulate size range and overall porosity on the resulting structure was evaluated. Results found 90% v/v scaffolds and particulate ranges above 100 µm to have the most optimal open framework and porosity. Resulting hydrophobic PDMS scaffolds were coated with fibronectin and evaluated as a platform for adherent cell culture using human mesenchymal stem cells. Biocompatibility of PDMS scaffolds was also evaluated in a rodent model, where implants were found to be highly biocompatibile and biostable, with positive extracellular matrix deposition throughout the scaffold. These results demonstrate the suitability of macroporous PDMS scaffolds for tissue engineering applications where strong integration with the host is desired. |
| Databáze: | OpenAIRE |
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