Adenosine receptors and second messenger signaling pathways in rat cardiac fibroblasts
| Autor: | Francisco Villarreal, Laurence L. Brunton, Sara Epperson, Israel Ramirez-Sanchez |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Male
medicine.medical_specialty Adrenergic Antagonists Physiology Extracellular Matrix Cell Interactions Biology GTP-Binding Protein alpha Subunits Gi-Go Receptor Adenosine A2B Second Messenger Systems Rats Sprague-Dawley Phosphoinositide Phospholipase C Fibrosis Receptors Adrenergic alpha-2 Internal medicine Receptors Adrenergic alpha-1 medicine Cyclic AMP Animals RNA Messenger Receptor Cells Cultured G protein-coupled receptor Mitogen-Activated Protein Kinase 1 Mitogen-Activated Protein Kinase 3 Myocardium Receptor Adenosine A3 Receptors Purinergic P1 Cell Biology Fibroblasts medicine.disease Adenosine Adenosine receptor Adrenergic Agonists Cyclic AMP-Dependent Protein Kinases Cell biology Extracellular Matrix Rats Endocrinology Second messenger system GTP-Binding Protein alpha Subunits Gq-G11 Myocardial fibrosis Collagen Signal transduction medicine.drug |
| Popis: | The ability of adenosine (ADO) to inhibit proliferation and protein synthesis (in particular, collagen synthesis) in cardiac fibroblasts (CF) may ameliorate adverse cardiac remodeling and fibrosis seen in heart failure patients. However, little is known about the signaling pathways that ADO may modulate in CF to alter cell phenotype. Accordingly, this study was designed to identify ADO receptors (AR) and the signaling pathways linked to them in primary cultures of adult rat CF. Quantitative RT-PCR data indicate that the mRNAs for all four known ARs (A1R, A2aR, A2bR, and A3R) are present in rat CF, with a greater prevalence of A2 receptor subtypes. No coupling of AR to the Gq-phospholipase C signaling pathway or to mobilization of calcium is measurable. Studies using subtype specific agents imply that the A2aR and A2bR couple to Gs-adenylyl cyclase and A1R couple weakly to Gi-adenylyl cyclase. 2-Chloroadenosine, 5'- N-ethylcarboxamidoadensoine, and other agents that elevate cellular cAMP stimulate extracellular signal-regulated kinase 1/2 activity in a pertussis toxin-insensitive manner. We conclude that a combination of cAMP-dependent signals generated via A2a and A2b receptors likely mediate ADO signaling in adult rat CF. |
| Databáze: | OpenAIRE |
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