Pressure Effect on the Nonradiative Process of Thioflavin-T
| Autor: | Nadav Amdursky, Rinat Gepshtein, Naum Koifman, Dan Huppert, Yuval Erez |
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| Rok vydání: | 2011 |
| Předmět: |
Time Factors
Molecular Structure Viscosity Hydrostatic pressure Analytical chemistry 1-Propanol Fluorescence Solvent Thiazoles chemistry.chemical_compound 1-Butanol Pentanols Nuclear magnetic resonance Reaction rate constant chemistry Pressure Thioflavin Benzothiazoles Physical and Theoretical Chemistry Linear correlation |
| Zdroj: | The Journal of Physical Chemistry A. 115:6481-6487 |
| ISSN: | 1520-5215 1089-5639 |
| DOI: | 10.1021/jp201537k |
| Popis: | Time-resolved emission techniques were employed to study the nonradiative process of thioflavin-T (ThT) in 1-propanol, 1-butanol, and 1-pentanol as a function of the hydrostatic pressure. Elevated hydrostatic pressure increases the alcohol viscosity, which in turn strongly influences the nonradiative rate of ThT. A diamond-anvil cell was used to increase the pressure up to 2.4 GPa. We found that the nonradiative rate constant, k(nr), decreases with pressure. We further found a remarkable linear correlation between a decrease in k(nr) (increase in the nonradiative lifetime, τ(nr)) and an increase in the solvent viscosity. The viscosity was varied by a factor of 1000 and k(nr) was measured at high pressures, at which the nonradiative rate constant of the molecules decreased from (7 ps)(-1) to (13 ns)(-1), (13 ps)(-1) to (17 ns)(-1) and (17 ps)(-1) to (15 ns)(-1) for 1-propanol, 1-butanol, and 1-pentanol, respectively. The viscosity-dependence of k(nr) is explained by the excited-state rotation rate of the two-ring systems, with respect to each other. |
| Databáze: | OpenAIRE |
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