LPS Response Is Impaired by Urban Fine Particulate Matter
| Autor: | Natália de Souza Xavier Costa, Gabriel Ribeiro Júnior, Adair Aparecida dos Santos Alemany, Luciano Belotti, Marcela Frota Cavalcante, Susan Ribeiro, Mariana Matera Veras, Esper Georges Kallás, Paulo Hilário Nascimento Saldiva, Marisa Dolhnikoff, Luiz Fernando Ferraz da Silva |
|---|---|
| Rok vydání: | 2022 |
| Předmět: |
Lipopolysaccharides
Male Mice Inbred BALB C Interleukin-6 Acute Lung Injury Interleukin-17 Organic Chemistry air pollution acute lung injury lipopolysaccharides inflammatory response General Medicine complex mixtures Catalysis Computer Science Applications Inorganic Chemistry Mice Animals Cytokines Particulate Matter Interleukin-4 Physical and Theoretical Chemistry Bronchoalveolar Lavage Fluid Lung Molecular Biology Spectroscopy |
| Zdroj: | International Journal of Molecular Sciences; Volume 23; Issue 7; Pages: 3913 |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms23073913 |
| Popis: | Fine particulate matter (PM2.5) is a complex mixture of components with diverse chemical and physical characteristics associated with increased respiratory and cardiovascular diseases mortality. Our study aimed to investigate the effects of exposure to concentrated PM2.5 on LPS-induced lung injury onset. BALB/c male mice were exposed to either filtered air or ambient fine PM2.5 in an ambient particle concentrator for 5 weeks. Then, an acute lung injury was induced with nebulized LPS. The animals were euthanized 24 h after the nebulization to either LPS or saline. Inflammatory cells and cytokines (IL-1β, IL-4, IL-5, IL-6, IL-10, IL-17, TNF) were assessed in the blood, bronchoalveolar lavage fluid (BALF), and lung tissue. In addition, lung morphology was assessed by stereological methods. Our results showed that the PM+LPS group showed histological evidence of injury, leukocytosis with increased neutrophils and macrophages, and a mixed inflammatory response profile, with increased KC, IL-6, IL-1β, IL-4, and IL-17. Our analysis shows that there is an interaction between the LPS nebulization and PM2.5 exposure, differently modulating the inflammatory response, with a distinct response pattern as compared to LPS or PM2.5 exposure alone. Further studies are required to explain the mechanism of immune modulation caused by PM2.5 exposure. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|