In vitro induction of secondary xylem-like tracheary elements in calli of hybrid poplar (Populus sieboldii × P. grandidentata)

Autor:	Joto Yoshimoto, Eri Nabeshima, Yusuke Yamagishi, Hiromu Uchiyama, Ryo Funada, Ugai Watanabe, Satoshi Nakaba
Rok vydání:	2013
Předmět:	chemistry.chemical_classification Microscopy Confocal Cellular differentiation fungi food and beverages Xylem Plant Science Biology In vitro Cell wall chemistry.chemical_compound Populus Reticulate chemistry Auxin Culture Techniques Hybrid poplar Botany Genetics Plant Vascular Bundle Brassinolide
Zdroj:	Planta. 237:1179-1185
ISSN:	1432-2048 0032-0935
Popis:	The formation of tracheary elements was induced in calli derived from petioles of hybrid poplar (Populus sieboldii × P. grandidentata) after 10 days of culture on medium that lacked auxin but contained 1 μM brassinolide. Some differentiated cells formed broad regions of cell walls and bordered pits, which are typical features of tracheary elements of secondary xylem. Other differentiated cells resembled tracheary elements of primary xylem, with spiral or reticulate thickening of cell walls. The tracheary elements that developed in calli were formed within cell clusters. This induction system provides a new model for studies of the mechanism of differentiation of secondary xylem cells in vitro.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a02017d2c7b2a0e5104e88b40447e194 https://doi.org/10.1007/s00425-013-1839-7 Zobrazit plný text záznamu Full text from SpringerLink