Psoriasin (S100A7) is a major Escherichia coli-cidal factor of the female genital tract
| Autor: | Johannes Pammer, Ramona Gmeiner, Leopold Eckhart, Michael Mildner, Erwin Tschachler, Martin Stichenwirth, Veronika Mlitz, C Sam, J-M Schröder, Regine Gläser, Arby Abtin, Alexandra Geusau |
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| Rok vydání: | 2010 |
| Předmět: |
S100A7
Adult Immunology Biology medicine.disease_cause Epithelium S100 Calcium Binding Protein A7 Microbiology Bacteriolysis Antigen Immunity medicine Escherichia coli Immunology and Allergy Humans Aged Antigens Bacterial Innate immune system S100 Proteins Genitalia Female Middle Aged biology.organism_classification In vitro Immunity Innate Gene Expression Regulation Vaginal Douching Female Bacteria Antimicrobial Cationic Peptides |
| Zdroj: | Mucosal immunology. 3(6) |
| ISSN: | 1935-3456 |
| Popis: | The female urogenital tract requires an efficient defense against bacteria, potentially derived from the adjacent intestinal tract. We have thus sought to identify the factors that protect against Escherichia coli (E. coli) in the female genital tract. Vaginal fluid from healthy human donors consistently killed E. coli in vitro and vaginal epithelium strongly expressed and secreted psoriasin. Psoriasin was constitutively produced in an organotypic vaginal epithelium model, and exposure of these cells to supernatants of E. coli cultures led to an enhanced psoriasin expression. Secreted psoriasin in vaginal fluids accounted for approximately 2.5-3% of total protein. Fractionation of vaginal fluids by high performance liquid chromatography (HPLC) showed that psoriasin co-eluted with a peak of E. coli killing activity. Our data show that normal vaginal fluid contains a powerful intrinsic antimicrobial defense against E. coli and that psoriasin contributes to the innate immune response of the female genital tract. |
| Databáze: | OpenAIRE |
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