Phylogenetic study of Baylisascaris schroederi isolated from Qinling subspecies of giant panda in China based on combined nuclear 5.8S and the second internal transcribed spacer (ITS-2) ribosomal DNA sequences
| Autor: | Una Ryan, Qing Lin, Man Gao, Hong-Mei Li, Wan-Xin Ren, Bing Hu, Guang-Hui Zhao, Mei-Mei Cong, Xing-Ye Wang, San-Ke Yu, Xing-Quan Zhu, Shui-Ping Zhang |
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| Rok vydání: | 2012 |
| Předmět: |
Genetic Markers
Male China Subspecies Homology (biology) Ascaridoidea DNA Ribosomal Spacer Animals Internal transcribed spacer Ribosomal DNA Phylogeny Phylogenetic tree biology Ecology Genetic Variation Bayes Theorem Sequence Analysis DNA DNA Helminth biology.organism_classification Maximum parsimony Infectious Diseases Evolutionary biology Genetic marker Ascarididae Female Parasitology Sequence Alignment Ursidae |
| Zdroj: | Parasitology International. 61:497-500 |
| ISSN: | 1383-5769 |
| DOI: | 10.1016/j.parint.2012.02.009 |
| Popis: | The nuclear ribosomal DNA (rDNA) region spanning 5.8S rDNA and the second internal transcribed spacer (ITS-2) of Baylisascaris schroederi isolated from the Qinling subspecies of giant panda in Shaanxi Province, China were amplified and sequenced. Sequence variations in the two rDNA regions within B. schroederi and among species in the family Ascarididae were examined. The lengths of B. schroederi 5.8S and ITS-2 rDNA sequences were 156. bp and 327. bp, respectively, and no nucleotide variation was found in these two rDNA regions among the 20 B. schroederi samples examined, and these ITS-2 sequences were identical to that of B. schroederi isolated from giant panda in Sichuan province, China. The inter-species differences in 5.8S and ITS-2 rDNA sequences among members of the family Ascarididae were 0-1.3% and 0-17.7%, respectively. Phylogenetic relationships among species in the Ascarididae were re-constructed by Bayesian inference (Bayes), maximum parsimony (MP), and maximum likelihood (ML) analyses, based on combined sequences of 5.8S and ITS-2 rDNA. All B. schroederi samples clustered together and sistered to B. transfuga with high posterior probabilities/bootstrap values, which further confirmed that nematodes isolated from the Qinling subspecies of giant panda in Shaanxi Province, China represent B. schroederi. Because of the large number of ambiguously aligned sequence positions (difficulty of inferring homology by positions), ITS-2 sequence alone is likely unsuitable for phylogenetic analyses at the family level, but the combined 5.8S and ITS-2 rDNA sequences provide alternative genetic markers for the identification of B. schroederi and for phylogenetic analysis of parasites in the family Ascarididae. |
| Databáze: | OpenAIRE |
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