The suppressive effect of tape-stripping treatment of guinea-pig skin on the induction of contact sensitivity by intradermal injection of haptenated epidermal cells
| Autor: | Hiroaki Ueki, Shojiro Nakagawa, Daisuke Oka |
|---|---|
| Rok vydání: | 1985 |
| Předmět: |
Male
Stripping (chemistry) Guinea Pigs Dermatology Pharmacology Dermatitis Contact Immune tolerance Guinea pig Dermis Dinitrochlorobenzene Immune Tolerance medicine Animals Intradermal injection Adenosine Triphosphatases Antiserum Phagocytes Chemistry Immunization Passive General Medicine Contact sensitivity medicine.anatomical_structure Immunization Langerhans Cells Immunology Epidermis Haptens |
| Zdroj: | Archives of Dermatological Research. 278:102-106 |
| ISSN: | 1432-069X 0340-3696 |
| DOI: | 10.1007/bf00409215 |
| Popis: | Contact sensitivity (CS) to 2,4-dinitrochlorobenzene (DNCB) was produced in inbred JY1-strain guinea pigs by the intradermal injection of epidermal cells (ECs) prepared from DNCB-painted skin (DNP-ECs). When the site of DNP-EC-induced CS was pretreated by tape stripping, the rate and intensity of the challenge reactions to DNCB were diminished. The ability of DNP-ECs to induce CS returned to normal when normal peritoneal macrophages together with DNP-ECs were administered into the stripped skin. Normal ECs had a similar effect. Using either anti-Ia antiserum and complement or allogeneic ECs (strains 2 and 13), Ia-positive cells among the ECs (presumably Langerhans cells) were found to be essential for the recovery of CS. Tape-stripping treatment also resulted in the development of immunological tolerance, as assessed by subsequent painting with a sensitizing dose of DNCB. These findings suggest that the immunological function of the mononuclear-phagocyte system in the dermis may be impaired when the epidermal surface is markedly disturbed by tape-stripping treatment. |
| Databáze: | OpenAIRE |
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