Dietary Fructo-Oligosaccharides Attenuate Early Activation of CD4+ T Cells Which Produce both Th1 and Th2 Cytokines in the Intestinal Lymphoid Tissues of a Murine Food Allergy Model
| Autor: | Akira Hosono, Mana Michimori, Masato Tsuda, Masanari Noda, Kyoko Takahashi, Narumi Ishii, Haruka Arakawa, Chihiro Ubukata, Shuichi Kaminogawa |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Lymphoid Tissue Ovalbumin medicine.medical_treatment T cell Immunology Oligosaccharides Mice Transgenic Fructose Lymphocyte Activation Mice 03 medical and health sciences Th2 Cells Antigen Food allergy medicine Animals Immunology and Allergy Mesenteric lymph nodes Cells Cultured Sensitization Mice Inbred BALB C biology Chemistry General Medicine Th1 Cells Mast cell medicine.disease Diet Intestines Disease Models Animal 030104 developmental biology medicine.anatomical_structure Cytokine biology.protein Cytokines Female Food Hypersensitivity |
| Zdroj: | International Archives of Allergy and Immunology. 174:121-132 |
| ISSN: | 1423-0097 1018-2438 |
| DOI: | 10.1159/000481984 |
| Popis: | Background: Fructo-oligosaccharides (FOS) are prebiotic agents with immunomodulatory effects involving improvement of the intestinal microbiota and metabolome. In this study, we investigated the cellular mechanisms through which FOS modulate intestinal antigen-specific CD4+ T cell responses in food allergy, using OVA23-3 mice. Methods: OVA23-3 mice were fed an experimental diet containing either ovalbumin (OVA) or OVA and FOS for 1 week. Body weight and mucosal mast cell protease 1 in the serum were measured as the indicator of intestinal inflammation. Single-cell suspensions were prepared from intestinal and systemic lymphoid tissues for cellular analysis. Cytokine production was measured by ELISA. Activation markers and intracellular cytokines in CD4+ T cells were analyzed by flow cytometry. Activated CD4+ T cells were purified to examine cytokine production. Results: Dietary intake of FOS provided moderate protection from the intestinal inflammation induced by the OVA-containing diet. FOS significantly reduced food allergy-induced Th2 cytokine responses in intestinal tissues but not in systemic tissues. FOS decreased OVA diet-induced IFN-γ+IL-4+ double-positive CD4+ T cells and early-activated CD45RBhighCD69+CD4+ T cells in the mesenteric lymph nodes. Furthermore, we confirmed that these CD45RBhighCD69+CD4+ T cells are able to produce high levels of IFN-γ and moderate level of IL-4, IL-10, and IL-13. Conclusions: Dietary intake of FOS during the development of food allergy attenuates the induction of intestinal Th2 cytokine responses by regulating early activation of naïve CD4+ T cells, which produce both Th1 and Th2 cytokines. Our results suggest FOS might be a potential food agent for the prevention of food allergy by modulating oral sensitization to food antigens. |
| Databáze: | OpenAIRE |
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