Organ-on-a-chip model of vascularized human bone marrow niches
| Autor: | Venktesh S. Shirure, Daniel C. Link, Drew Elizabeth Glaser, Natalie R. Ng, Leif S. Anderson, Matthew B. Curtis, Peter A. Sariano, Zachary A. Rollins, Aravind Anand, Steven C. George, Alyssa M. Deely, Jeremy M. Lowen, Bhupinder S. Shergill, Katherine N. Weilbaecher |
|---|---|
| Rok vydání: | 2022 |
| Předmět: |
Myeloid
Niche Biophysics CD34 Bone Marrow Cells Bioengineering Biology Organ-on-a-chip Bone and Bones Biomaterials 03 medical and health sciences 0302 clinical medicine Tissue engineering Bone Marrow Lab-On-A-Chip Devices medicine Humans Stem Cell Niche Progenitor cell 030304 developmental biology 0303 health sciences Intravasation Cell Differentiation Hematopoietic Stem Cells Hematopoiesis Cell biology Haematopoiesis medicine.anatomical_structure Mechanics of Materials 030220 oncology & carcinogenesis Ceramics and Composites Bone marrow Stem cell |
| Zdroj: | Biomaterials. 280:121245 |
| ISSN: | 0142-9612 |
| DOI: | 10.1016/j.biomaterials.2021.121245 |
| Popis: | Animal models of bone marrow have limited spatial and temporal resolution to observe biological events (intravasation and cellular egress) and are inadequate to dissect dynamic events at the niche level (100 microns). Utilizing microfluidic and stem cell technology, we present a 3D in vitro model of human bone marrow that contains perivascular and endosteal niches complete with dynamic, perfusable vascular networks. We demonstrate that our model can perform in vivo functions including maintenance and differentiation of CD34+ hematopoietic stem/progenitor cells (HSPC) for up to fourteen days, egress of myeloid progenitors, and expression of markers consistent with in vivo human bone marrow. The platform design enables the addition of tissue niches at a later timepoint to probe mechanisms such as tumor cell migration. Overall, we present a novel organ-on-a-chip platform that is capable of recapitulating the human bone marrow microenvironment to observe hematopoietic phenomena at high spatial and temporal resolution. |
| Databáze: | OpenAIRE |
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