Cytokinin oxidase PpCKX1 plays regulatory roles in development and enhances dehydration and salt tolerance in Physcomitrella patens
| Autor: | Mei Hua Cui, Sung Hyun Cho, Sujin Hyoung, Won Mi So, Joo Hee Chung, Jeong Sheop Shin |
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| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Cytokinins Cell division Plant Science Vacuole Physcomitrella patens 01 natural sciences Salt Stress 03 medical and health sciences chemistry.chemical_compound Gene Expression Regulation Plant Phylogeny biology Dehydration Stem Cells fungi food and beverages General Medicine Salt Tolerance biology.organism_classification Plants Genetically Modified Phenotype Bryopsida Cell biology 030104 developmental biology Rhizoid chemistry Shoot Cytokinin Vacuoles Stem cell Oxidoreductases Agronomy and Crop Science 010606 plant biology & botany |
| Zdroj: | Plant cell reports. 39(3) |
| ISSN: | 1432-203X |
| Popis: | PpCKX1 localizes to vacuoles and is dominantly expressed in the stem cells. PpCKX1 regulates developmental changes with increased growth of the rhizoid and enhances dehydration and salt tolerance. Cytokinins (CKs) are plant hormones that regulate plant development as well as many physiological processes, such as cell division, leaf senescence, control of shoot/root ratio, and reproductive competence. Cytokinin oxidases/dehydrogenases (CKXs) control CK concentrations by degradation, and thereby influence plant growth and development. In the moss Physcomitrella patens, an evolutionarily early divergent plant, we identified six putative CKXs that, by phylogenetic analysis, form a monophyletic clade. We also observed that ProPpCKX1:GUS is expressed specifically in the stem cells and surrounding cells and that CKX1 localizes to vacuoles, as indicated by Pro35S:PpCKX1-smGFP. Under normal growth conditions, overexpression of PpCKX1 caused many phenotypic changes at different developmental stages, and we suspected that increased growth of the rhizoid could affect those changes. In addition, we present evidence that the PpCKX1-overexpressor plants show enhanced dehydration and salt stress tolerance. Taken together, we suggest that PpCKX1 plays regulatory roles in development and adaptation to abiotic stresses in this evolutionarily early land plant species. |
| Databáze: | OpenAIRE |
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