Effect of chelated iron activated peroxydisulfate oxidation on perchloroethene-degrading microbial consortium
Autor: | Tereza Smrhova, Jiri Marek, Marek Martinec, Ondrej Uhlik, Radek Škarohlíd, Michal Strejcek, Zuzana Roskova, Lenka McGachy |
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Rok vydání: | 2021 |
Předmět: |
Tetrachloroethylene
Environmental Engineering Iron Health Toxicology and Mutagenesis Microbial Consortia 0208 environmental biotechnology 02 engineering and technology 010501 environmental sciences 01 natural sciences chemistry.chemical_compound RNA Ribosomal 16S Peroxydisulfate Environmental Chemistry 0105 earth and related environmental sciences biology Brevundimonas Pseudomonas Public Health Environmental and Occupational Health food and beverages General Medicine General Chemistry Microbial consortium biology.organism_classification Pollution 020801 environmental engineering Biodegradation Environmental chemistry In situ chemical oxidation Citric acid Microcosm Water Pollutants Chemical Bacteria Nuclear chemistry |
Zdroj: | Chemosphere. 266:128928 |
ISSN: | 0045-6535 |
Popis: | In this work, the effect of In-Situ Chemical Oxidation (ISCO) using peroxydisulfate (PDS) on chloroethenes-degrading microbial consortium in the presence of perchloroethene (PCE; tetrachloroethene) was investigated. Degradation of PCE was examined using PDS without an activation, activated with iron Fe(II) chelated by citric acid (CA), and microbial consortium derived from chloroethenes-contaminated site in liquid and sand microcosms. Two different molar ratios of PCE/PDS/(Fe(II)+CA) (1/8/1.6 and 1/16/3.2) were tested. The PCE removal efficiency was the highest in the bacteria-free microcosms. An expected increase in the PCE removal efficiency by coupling PDS and microbial consortium was not confirmed. Surprisingly, the reduced capacity of PDS to remove PCE in the systems containing both PDS and microbial consortium was observed indicating that indigenous microbes may reduce the efficiency of PDS during a remediation. High-throughput 16S rRNA gene sequencing analysis revealed negative effect of PDS on organohalide-respiring bacteria (OHRB), which were not detected after 19 days of the experiment, unlike in biotic control. On the other hand, amplicon sequence variants (ASVs) affiliated with genera Brevundimonas and Pseudomonas that have been described for their capability of aerobic cometabolic/metabolic degradation of chloroethenes (CEs) were among the most frequently detected ASVs after the PDS treatment. Results further showed that the sole Fe(II)-CA affected the diversity of the microbial consortium. Overall, results of this study provide new insight into the coupling ISCO using PDS with in situ bioremediation of CEs. |
Databáze: | OpenAIRE |
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