Investigation of Metabolism of Exogenous Glucose at the Early Stage and Onset of Diabetes Mellitus in Otsuka Long-Evans Tokushima Fatty Rats Using [1, 2, 3-13C]Glucose Breath Tests

Autor: Sho Kijima, Hideki Tanaka, Yoshihisa Urita, Osamu Kano, Masaaki Takayanagi, Naoyuki Kawagoe
Rok vydání: 2015
Předmět:
0301 basic medicine
Metabolic Processes
Time Factors
Physiology
lcsh:Medicine
Biochemistry
0302 clinical medicine
Endocrinology
Glucose Metabolism
Medicine and Health Sciences
Prediabetes
lcsh:Science
Glucose tolerance test
Carbon Isotopes
Multidisciplinary
medicine.diagnostic_test
Chemistry
Organic Compounds
Monosaccharides
Chemical Reactions
Breath Tests
Exhalation
Physical Sciences
Carbohydrate Metabolism
Research Article
medicine.medical_specialty
Endocrine Disorders
Rats
Inbred OLETF
Citric Acid Cycle
Excretion
Carbohydrates
030209 endocrinology & metabolism
Carbohydrate metabolism
Carbon Cycle
03 medical and health sciences
Insulin resistance
Internal medicine
Diabetes mellitus
Oxidation
medicine
Diabetes Mellitus
Animals
Humans
Environmental Chemistry
Rats
Long-Evans
Breath test
Endocrine Physiology
lcsh:R
Body Weight
Organic Chemistry
Ecology and Environmental Sciences
Chemical Compounds
Biology and Life Sciences
Metabolism
Carbon Dioxide
Glucose Tolerance Test
medicine.disease
Rats
Disease Models
Animal
030104 developmental biology
Glucose
Diabetes Mellitus
Type 2
Metabolic Disorders
lcsh:Q
Insulin Resistance
Physiological Processes
Zdroj: PLoS ONE
PLoS ONE, Vol 11, Iss 8, p e0160177 (2016)
ISSN: 1932-6203
Popis: This study aimed to evaluate changes in glucose metabolism at the early stage and onset of diabetes in Otsuka Long-Evans Tokushima Fatty (OLETF) rats. Specifically, after the oral administration of [1, 2, 3-13C]glucose, the levels of exhaled 13CO2, which most likely originated from pyruvate decarboxylation and tricarboxylic acid, were measured. Eight OLETF rats and eight control rats (Long-Evans Tokushima Otsuka [LETO]) were administered 13C-glucose. Three types of 13C-glucose breath tests were performed thrice in each period at 2-week intervals. [3-13C]glucose results in a 13C isotope at position 1 in the pyruvate molecule, which provides 13CO2. The 13C at carbons 1 and 2 of glucose is converted to 13C at carbons 2 and 1 of acetate, respectively, which produce 13CO2. Based on metabolic differences of the labeled sites, glucose metabolism was evaluated using the results of three breath tests. The increase in 13CO2 excretion in OLETF rats was delayed in all three breath tests compared to that in control rats, suggesting that OLETF rats had a lower glucose metabolism than control rats. In addition, overall glucose metabolism increased with age in both groups. The utilization of [2-13C]glucose was suppressed in OLETF rats at 6-12 weeks of age, but they showed higher [3-13C]glucose oxidation than control rats at 22-25 weeks of age. In the [1-13C]glucose breath test, no significant differences in the area under the curve until 180 minutes (AUC180) were observed between OLETF and LETO rats of any age. Glucose metabolism kinetics were different between the age groups and two groups of rats; however, these differences were not significant based on the overall AUC180 of [1-13C]glucose. We conclude that breath 13CO2 excretion is reduced in OLETF rats at the primary stage of prediabetes, indicating differences in glucose oxidation kinetics between OLETF and LETO rats.
Databáze: OpenAIRE
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