Optoelectrofluidic enhanced immunoreaction based on optically-induced dynamic AC electroosmosis
| Autor: | Je-Kyun Park, Dongsik Han |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Optics and Photonics
Analyte Surface Properties Biomedical Engineering Analytical chemistry Bioengineering 02 engineering and technology 01 natural sciences Biochemistry Antibodies Effective mass (solid-state physics) Binding efficiency Lab-On-A-Chip Devices Reaction chamber Immunoassay Chemistry Photoconductivity 010401 analytical chemistry Electric Conductivity Electrochemical Techniques General Chemistry 021001 nanoscience & nanotechnology 0104 chemical sciences Indium tin oxide Electroosmosis 0210 nano-technology Mathematical simulation Voltage |
| Zdroj: | Lab on a Chip. 16:1189-1196 |
| ISSN: | 1473-0189 1473-0197 |
| DOI: | 10.1039/c6lc00110f |
| Popis: | We report a novel optoelectrofluidic immunoreaction system based on electroosmotic flow for enhancing antibody-analyte binding efficiency on a surface-based sensing system. Two conventional indium tin oxide glass slides are assembled to provide a reaction chamber for a tiny volume of sample droplet (∼5 μL), in which the top layer is employed as an antibody-immobilized substrate and the bottom layer acts as a photoconductive layer of an optoelectrofluidic device. Under the application of an AC voltage, an illuminated light pattern on the photoconductive layer causes strong counter-rotating vortices to transport analytes from the bulk solution to the vicinity of the assay spot on the glass substrate. This configuration overcomes the slow immunoreaction problem of a diffusion-based sensing system, resulting in the enhancement of binding efficiency via an optoelectrofluidic method. Furthermore, we investigate the effect of optically-induced dynamic AC electroosmotic flow on optoelectrofluidic enhancement for surface-based immunoreaction with a mathematical simulation study and real experiments using immunoglobulin G (IgG) and anti-IgG. As a result, dynamic light patterns provided better immunoreaction efficiency than static light patterns due to effective mass transport of the target analyte, resulting in an achievement of 2.18-fold enhancement under a growing circular light pattern compared to the passive mode. |
| Databáze: | OpenAIRE |
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