PSVII-20 Differential gene expression of day 15 endometrium of pregnant Bos indicus beef cows according to expression of estrus at timed-AI
| Autor: | Reinaldo F Cooke, R. S. Cipriano, P. F. M. P. Souto, B.F. Silper, Ronaldo L.A. Cerri |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Estrous cycle
endocrine system urogenital system 0402 animal and dairy science food and beverages 04 agricultural and veterinary sciences General Medicine Biology Endometrium 040201 dairy & animal science Andrology Abstracts medicine.anatomical_structure Gene expression 040103 agronomy & agriculture Genetics medicine 0401 agriculture forestry and fisheries Animal Science and Zoology reproductive and urinary physiology Food Science |
| Popis: | Our objective was to compare the expression of target genes in the endometrium of 15-d pregnant cows that expressed estrus at timed-AI (EST) with those that did not express estrus (NOEST). Multiparous Nelore cows (n = 100) were inseminated by the same technician with semen from a single bull on d 0, following a progesterone-estradiol protocol (d -11 to 0). Estrus expression assessed on d 0 was positive if > 50% of a estrus detection patch rub-off coating attached to the cow’s tailhed on d -2 had been removed. On d 15, 60 cows that had a corpus luteum (CL) > 0.38 cmP < 0.10) to be greater for EST than NOEST cows (2.5 ± 0.5 vs. 1.2 ± 0.5 cm). Downregulation was observed in EST cows for genes related to steroid synthesis (HPDG, PGR, ERa, CYP4X1), embryonic development (ANIX2, MOGAT1, GSK3b, VEGFA, APC, PFKFB2), and nutrient transport and metabolism (FOLR1, NNMT). Immune-related gene MUC1 was upregulated and IL10 was downregulated in EST cows. Adhesion genes CTNNA2 and MYH9 were down and upregulated in EST cows, respectively. These result evidence differential gene expression on d 15 endometrium of pregnant cows according to estrus expression at timed-AI, potentially supporting maintenance of pregnancy via mechanisms of luteolysis inhibition, enhanced adhesion, and reduction of Wnt pathway negative effectors favoring embryo development. |
| Databáze: | OpenAIRE |
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