Endoplasmic reticulum stress regulates mechanical stress-induced ossification of posterior longitudinal ligament
| Autor: | Lei Shi, Jinhao Miao, Yu Chen, Jiangang Shi, Deyu Chen |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Adult
Male MAPK/ERK pathway Ossification of Posterior Longitudinal Ligament eIF-2 Kinase 03 medical and health sciences 0302 clinical medicine In vivo medicine Humans Posterior longitudinal ligament Orthopedics and Sports Medicine RNA Messenger Endoplasmic Reticulum Chaperone BiP Heat-Shock Proteins 030222 orthopedics biology Ossification business.industry Endoplasmic reticulum Cell Differentiation Fibroblasts Middle Aged Endoplasmic Reticulum Stress Longitudinal Ligaments Cell biology medicine.anatomical_structure Case-Control Studies Mitogen-activated protein kinase Ligament Unfolded protein response biology.protein Female Surgery Stress Mechanical Mitogen-Activated Protein Kinases medicine.symptom business Biomarkers Transcription Factor CHOP 030217 neurology & neurosurgery |
| Zdroj: | European Spine Journal. 28:2249-2256 |
| ISSN: | 1432-0932 0940-6719 |
| DOI: | 10.1007/s00586-019-06074-2 |
| Popis: | The pathogenesis of ossification of posterior longitudinal ligament (OPLL) is not completely clear. Previous study has confirmed a single-pass type I endoplasmic reticulum (ER) membrane protein kinase (PERK), which is a major transducer of the ER stress, participates in the process of OPLL in vitro. This study aimed to demonstrate the role of ER stress in mechanical stress (MS)-induced OPLL. The posterior longitudinal ligaments were collected intraoperatively. The expression of ER stress markers in ligament tissue samples was compared between OPLL and non-OPLL patients in vivo. Ligament fibroblasts were isolated and cultured. Loaded by MS, the expression of ER stress markers in fibroblasts deriving from non-ossified areas of the ligament tissues from OPLL patients was detected. The influence of inhibition of ER stress on MS-induced OPLL and activation of mitogen-activated protein kinase (MAPK) pathways by MS was also investigated. We confirmed the ER stress markers were highly expressed in non-ossified areas of the ligament tissues from OPLL patients but could barely be detected in the ligaments from non-OPLL patients in vivo. We also found ER stress could be activated by MS during the process of OPLL in vitro. Moreover, inhibition of ER stress could hinder MS-induced OPLL and activation of MAPK signaling pathways by MS in vitro. Activated ER stress was observed in OPLL patients both in vitro and in vivo. Mechanical stress could activate ER stress response in posterior longitudinal ligament fibroblasts and further promote OPLL in vitro. In this process, ER stress might work through the MAPK signaling pathways. These slides can be retrieved under Electronic Supplementary Material. |
| Databáze: | OpenAIRE |
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