Expression of the apoptosis-related proteins caspase-3 and NF-kappaB in the hippocampus of Tg2576 mice
| Autor: | Dong-Ming Yu, Jin-Bo Deng, Wei-Juan Zhang, Bin Liu, Guo-Tao Sun, Ping Wu, Yan-Li Niu |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
medicine.medical_specialty
Aging Physiology Transgene Hippocampus Caspase 3 Apoptosis Cell Count Mice Transgenic Biology chemistry.chemical_compound Mice Alzheimer Disease Internal medicine medicine Animals RNA Messenger Neurons General Neuroscience Pyramidal Cells NF-kappa B RNA NF-κB General Medicine Human physiology medicine.disease CA3 Region Hippocampal Cell biology Mice Inbred C57BL Disease Models Animal Endocrinology chemistry Animals Newborn Original Article Alzheimer's disease |
| Zdroj: | Neuroscience bulletin. 26(1) |
| ISSN: | 1995-8218 |
| Popis: | To investigate the relations between neuroapoptosis and the onset and development of Alzheimer's disease (AD), especially the role of NF-kappaB in the regulation of neuroapoptosis.Caspase-3 and NF-kappaB (p50) expressions in the CA3 region of the hippocampus in APPswe Tg2576 transgenic mice were studied from postnatal day 0-180, using Nissl staining, immunohistochemistry and RT-PCR methods.Both neuronal apoptosis and NF-kappaB activity decreased gradually with the increase of age in wild type and Tg2576 mice. However, the number of caspase-3-positive or NF-kappaB-positive pyramidal cells in Tg2576 mice was greater than that in age-matched wild type mice, with significant differences after postnatal day 14 (P0.01 or P0.05). Linear regression analyses of caspase-3 and NF-kappaB expression demonstrated a correlation between neuroapoptosis and activity of NF-kappaB.The process of neuroapoptosis is consistent with the onset and development of AD. Furthermore, the observed correlation between neuroapoptosis and NF-kappaB activity suggests a role of NF-kappaB in hippocampal neuroapoptosis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink