In Vitro Selection of RNA Aptamers to a Protein Target by Filter Immobilization
| Autor: | Catherine Bowman, Bradley Hall, Seyed Arshad, Andrew D. Ellington, Sulay D. Jhaveri, Kyunghyun Seo, Meredith Corley |
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| Rok vydání: | 2010 |
| Předmět: |
Chemistry
Aptamer Ligand binding assay Organic Chemistry SELEX Aptamer Technique DNA Single-Stranded RNA Proteins Aptamers Nucleotide Polymerase Chain Reaction Biochemistry Reverse transcriptase In vitro Affinity Reagent Nucleic acid Indicators and Reagents Directed Molecular Evolution Molecular Biology Filtration Systematic evolution of ligands by exponential enrichment Protein Binding |
| Zdroj: | Current Protocols in Molecular Biology. 89 |
| ISSN: | 1934-3647 1934-3639 |
| DOI: | 10.1002/0471142727.mb2403s88 |
| Popis: | This unit describes the selection of aptamers from a pool of single-stranded RNA by binding to a protein target. Aptamers generated from this selection experiment can potentially act as protein function inhibitors, and may find applications as therapeutic or diagnostic reagents. A pool of dsDNA is used to generate an ssRNA pool, which is mixed with the protein target. Bound complexes are separated from unbound reagents by filtration, and the RNA:protein complexes are amplified by a combination of reverse transcription, PCR, and in vitro transcription. Curr. Protoc. Nucleic Acid Chem. 40:9.3.1-9.3.27. © 2010 by John Wiley & Sons, Inc. Keywords: aptamer; in vitro selection; affinity reagent; filter binding assay; SELEX |
| Databáze: | OpenAIRE |
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