The PTS transporters of Lactobacillus gasseri ATCC 33323
| Autor: | Alyssa L. Francl, Taksawan Thongaram, Michael J. Miller |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Microbiology (medical)
Sucrose lcsh:QR1-502 Mannose macromolecular substances Cellobiose Carbohydrate metabolism Lactobacillus gasseri Polymerase Chain Reaction Microbiology lcsh:Microbiology Phosphotransferase chemistry.chemical_compound Research article otorhinolaryngologic diseases Gene Silencing Phosphoenolpyruvate Sugar Phosphotransferase System Cell Proliferation biology Gene Expression Profiling Computational Biology Genetic Variation Fructose Carbohydrate biology.organism_classification carbohydrates (lipids) stomatognathic diseases Lactobacillus Mutagenesis Insertional chemistry Biochemistry bacteria Carbohydrate Metabolism |
| Zdroj: | BMC Microbiology BMC Microbiology, Vol 10, Iss 1, p 77 (2010) |
| ISSN: | 1471-2180 |
| DOI: | 10.1186/1471-2180-10-77 |
| Popis: | Background Lactobacilli can utilize a variety of carbohydrates which reflects the nutrient availability in their respective environments. A common lactobacilli in the human gastrointestinal tract, Lactobacillus gasseri, was selected for further study. The currently available annotation of the L. gasseri ATCC 33323 genome describes numerous putative genes involved in carbohydrate utilization, yet the specific functions of many of these genes remain unknown. Results An enzyme I (EI) knockout strain revealed that a functional phosphotransferase transporter system (PTS) is required to ferment at least 15 carbohydrates. Analysis of the L. gasseri ATCC 33323 genome identified fifteen complete (containing all of the necessary subunits) PTS transporters. Transcript expression profiles in response to various carbohydrates (glucose, mannose, fructose, sucrose and cellobiose) were analyzed for the fifteen complete PTS transporters in L. gasseri. PTS 20 was induced 27 fold in the presence of sucrose and PTS 15 was induced 139 fold in the presence of cellobiose. No PTS transporter was induced by glucose, fructose or mannose. Insertional inactivation of PTS 15 and PTS 20 significantly impaired growth on cellobiose and sucrose, respectively. As predicted by bioinformatics, insertional inactivation of PTS 21 confirmed its role in mannose utilization. Conclusions The experiments revealed the extensive contribution of PTS transporters to carbohydrate utilization by L. gasseri ATCC 33323 and the general inadequacy of the annotated sugar specificity of lactobacilli PTS transporters. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|