Visualization of degenerating axons in a dysmyelinating mouse mutant with axonal loss
| Autor: | Birgit Ey, Antje Kroner, Rudolf Martini, Heinrich Blazyca, Igor Kobsar |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Yellow fluorescent protein
Immunoelectron microscopy Axonal loss Schwann cell Quadriceps Muscle Mice Cellular and Molecular Neuroscience Myelin Phagocytosis medicine Fluorescence microscope Animals Axon Microscopy Immunoelectron Molecular Biology Mice Knockout biology Macrophages Cell Biology Sciatic Nerve Axons Cell biology Mice Inbred C57BL Luminescent Proteins medicine.anatomical_structure Microscopy Fluorescence nervous system Axoplasm Nerve Degeneration biology.protein Schwann Cells Tibial Nerve Myelin P0 Protein Neuroscience |
| Zdroj: | Molecular and Cellular Neuroscience. 35:153-160 |
| ISSN: | 1044-7431 |
| Popis: | Mice homozygously deficient for the myelin component P0 show loss of axons in peripheral nerves. In order to investigate the morphological characteristics of degenerating axons, we crossbred the myelin mutants with a transgenic mouse line expressing yellow fluorescent protein (YFP) in a small proportion of neurons. Peripheral nerves of the double mutants were prepared into small fiber bundles and investigated by fluorescence microscopy. We could identify the tips of degenerating axon as bulb-like structures. Additionally, by electron microscopy, these structures were characterized as axoplasmic extensions containing numerous membraneous compartments. By immunoelectron microscopy, the degenerating end bulbs were in contact with ensheathing Schwann cells that contained YFP-immunoreactivity possibly reflecting phagocytosis of axon material by these cells. Immunohistochemistry using antibodies against macrophages revealed that YFP-positive bulbs, but also other axonal swellings, were often associated with macrophages supporting our previous findings that myelin-related axonal loss is partially mediated by these cells. |
| Databáze: | OpenAIRE |
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