High-throughput cellular screening of engineered ECM based on combinatorial polyelectrolyte multilayer films
| Autor: | Miloslav Sailer, Ozzy Mermut, Karen Lai Wing Sun, Christopher J. Barrett, Timothy E. Kennedy |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Scaffold
Materials science Biocompatibility Cell Survival Acrylic Resins Biophysics Bioengineering Nanotechnology 02 engineering and technology 010402 general chemistry 01 natural sciences Cell Line Biomaterials Extracellular matrix Coated Materials Biocompatible Tissue engineering Materials Testing Polyamines Animals Humans Viability assay Cells Cultured Neurons HEK 293 cells Cell Differentiation Equipment Design 021001 nanoscience & nanotechnology Polyelectrolyte Extracellular Matrix High-Throughput Screening Assays Rats 0104 chemical sciences Transplantation HEK293 Cells Mechanics of Materials Ceramics and Composites 0210 nano-technology |
| Zdroj: | Biomaterials. 33:5841-5847 |
| ISSN: | 0142-9612 |
| DOI: | 10.1016/j.biomaterials.2012.05.001 |
| Popis: | The capacity to engineer the extracellular matrix is critical to better understand cell function and to design optimal cellular environments to support tissue engineering, transplantation and repair. Stacks of adsorbed polymers can be engineered as soft wet three dimensional matrices, with properties tailored to support cell survival and growth. Here, we have developed a combinatorial method to generate coatings that self assemble from solutions of polyelectrolytes in water, layer by layer, to produce a polyelectrolyte multilayer (PEM) coating that has enabled high-throughput screening for cellular biocompatibility. Two dimensional combinatorial PEMs were used to rapidly identify assembly conditions that promote optimal cell survival and viability. Conditions were first piloted using a cell line, human embryonic kidney 293 cells (HEK 293), and subsequently tested using primary cultures of embryonic rat spinal commissural neurons. Cell viability was correlated with surface energy (wettability), modulus (matrix stiffness), and surface charge of the coatings.Our findings indicate that the modulus is a crucial determinant of the capacity of a surface to inhibit or support cell survival. |
| Databáze: | OpenAIRE |
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