Enzyme assays using sensor arrays based on ion-selective carbon nanotube field-effect transistors
| Autor: | Katharina Melzer, Krzysztof Maksymiuk, V. Deep Bhatt, R. Mittermeier, Agata Michalska, Ewa Jaworska, Paolo Lugli |
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| Rok vydání: | 2016 |
| Předmět: |
Analyte
Materials science Transistors Electronic Biomedical Engineering Biophysics Nanotechnology Biosensing Techniques 02 engineering and technology Carbon nanotube 01 natural sciences Ion law.invention law Electrochemistry Humans Urea Enzyme Assays biology Nanotubes Carbon 010401 analytical chemistry Transistor Equipment Design General Medicine Enzymes Immobilized 021001 nanoscience & nanotechnology Urease Enzyme assay 0104 chemical sciences Membrane biology.protein Surface modification Field-effect transistor 0210 nano-technology Biotechnology |
| Zdroj: | Biosensors and Bioelectronics. 84:7-14 |
| ISSN: | 0956-5663 |
| DOI: | 10.1016/j.bios.2016.04.077 |
| Popis: | In the fields of clinical diagnostics and point-of-care diagnosis as well as food and environmental monitoring there is a high demand for reliable high-throughput, rapid and highly sensitive assays for a simultaneous detection of several analytes in complex and low-volume samples. Sensor platforms based on solution-processable electrolyte-gated carbon nanotube field-effect transistors (CNT-FETs) are a simple and cost-effective alternative for conventional assays. In this work we demonstrate a selective as well as direct detection of the products of an enzyme-substrate interaction, here the for metabolic processes important urea-urease system, with sensors based on spray-coated CNT-FETs. The selective and direct detection is achieved by immobilizing the enzyme urease via certain surface functionalization techniques on the sensor surface and further modifying the active interfaces with polymeric ion-selective membranes as well as pH-sensitive layers. Thereby, we can avoid the generally applied approach for a field-effect based detection of enzyme reactions via detecting changes in the pH value due to an on-going enzymatic reaction and directly detect selectively the products of the enzymatic conversion. Thus, we can realize a buffering-capacity independent monitoring of changes in the substrate concentration. |
| Databáze: | OpenAIRE |
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