Identification and Sequencing of Bacterial rDNAs in Culture-Negative Amniotic Fluid from Women in Premature Labor
Autor: | Donald E. Riley, Jane Hitti, David A. Eschenbach, Carolyn Gardella, John N. Krieger, Kathy Agnew |
---|---|
Rok vydání: | 2004 |
Předmět: |
Adult
DNA Bacterial Amniotic fluid Biology medicine.disease_cause DNA Ribosomal Polymerase Chain Reaction law.invention Microbiology Proinflammatory cytokine Obstetric Labor Premature Pregnancy law RNA Ribosomal 16S medicine Humans Ribosomal DNA Polymerase chain reaction Interleukin-6 Tumor Necrosis Factor-alpha Obstetrics and Gynecology Amniotic Fluid biology.organism_classification 16S ribosomal RNA Pediatrics Perinatology and Child Health Female Fusobacterium nucleatum Gram-Negative Bacterial Infections Bacteria Ureaplasma urealyticum |
Zdroj: | American Journal of Perinatology. 21:319-323 |
ISSN: | 1098-8785 0735-1631 |
Popis: | Our objective was to identify bacterial species present in culture-negative but 16S rDNA-positive amniotic fluid samples from women in preterm labor. Amniotic fluid from 69 women in preterm labor was cultured and examined for the proinflammatory cytokine interleukin-6 (IL-6). Polymerase chain reaction technology was used to detect highly conserved bacterial ribosomal DNA sequences (16S rDNAs). As previously reported, 16S rDNAs were identified in 15 (94%) of 16 culture-positive amniotic fluid samples, in 5 (36%) of 14 culture-negative samples with elevated IL-6, and in 1 (3%) of 39 culture-negative samples with low IL-6 levels. Direct sequencing was performed of 16S rDNAs from the 5 culture-negative amniotic fluid specimens with elevated IL-6, followed by database searches and phylogenetic analyses. The bacterial sequences identified included: two Leptotrichia sanguinegens, one human oral bacterium A33, one Fusobacterium nucleatum, and one Ureaplasma urealyticum. Identification and sequencing of 16S rDNAs in amniotic fluid is a promising technique to identify bacterial species associated with elevated IL-6 levels in culture-negative amniotic fluid that may contribute to the etiology of premature labor. |
Databáze: | OpenAIRE |
Externí odkaz: |