Snake Venom Hemotoxic Enzymes: Biochemical Comparison between Crotalus Species from Central Mexico

Autor:	Alejandro Blanco-Labra, Martha Sandra Cruz-Pérez, Teresa García-Gasca, Luis Fernando Díaz-Peña, Carlos Saldaña, José Alejandro García-Arredondo, Octavio Roldán-Padrón, José Luis Castro-Guillén
Rok vydání:	2019
Předmět:	serine proteases Proteases Pharmaceutical Science Venom complex mixtures phospholipases A2 Analytical Chemistry lcsh:QD241-441 03 medical and health sciences lcsh:Organic chemistry Crotalus aquilus Drug Discovery metalloproteases Physical and Theoretical Chemistry snake venom 030304 developmental biology 0303 health sciences biology Crotalus 030302 biochemistry & molecular biology Organic Chemistry hyaluronidases biology.organism_classification Hemotoxin Crotalus polystictus Biochemistry Chemistry (miscellaneous) Snake venom Molecular Medicine Crotalus molossus
Zdroj:	Molecules, Vol 24, Iss 8, p 1489 (2019) Molecules Volume 24 Issue 8
ISSN:	1420-3049
DOI:	10.3390/molecules24081489
Popis:	Snakebite envenoming is a serious medical problem in different areas of the world. In Latin America, the major prevalence is due to snakes of the family Viperidae, where rattlesnakes (Crotalus) are included. They produce hemotoxic venom which causes bleeding, tissue degradation and necrosis. Each venom has several enzymatic activities, producing different effects in the envenoming, doing its clinical effects difficult to study. Comparison between venom molecules is also difficult when different techniques are used, and therefore, their identification/characterization using the same methodology is necessary. In this work, a general biochemical characterization in snake venom of serine proteases (SVSP), phospholipases A2 (PLA2), metalloproteases (SVMP) and hyaluronidases (SVH) of Crotalus aquilus (Ca), Crotalus polystictus (Cp) and Crotalus molossus nigrescens (Cmn) was done. Differences in protein pattern, enzyme content and enzymatic activities were observed. All the venoms showed high PLA2 activity, high molecular weight SVSP, and a wide variety of SVMP and SVH forms. Ca and Cp showed the highest enzymatic activities of SVMP and SVSP trypsin-like and chymotrypsin-like, whereas Cmn showed the highest SVH and similar PLA2 activity with Ca. All the venoms showed peptides with similar molecular weight to crotamine-like myotoxins. No previous biochemical characterization of C. aquilus has been reported and there are no previous analyses that include these four protein families in these Crotalus venoms.
Databáze:	OpenAIRE
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