MYCN silencing induces differentiation and apoptosis in human neuroblastoma cells
| Autor: | B. Mark Evers, Jingbo Qiao, Titilope A. Ishola, Dai H. Chung, Piotr G. Rychahou, Jung Hee Kang |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Small interfering RNA
Cellular differentiation Biophysics Apoptosis Biology Biochemistry Article Cell Line Neuroblastoma Downregulation and upregulation RNA interference Gene expression medicine Humans Gene silencing Gene Silencing neoplasms Molecular Biology Neurons Oncogene Proteins N-Myc Proto-Oncogene Protein Cell growth Nuclear Proteins Cell Differentiation Cell Biology medicine.disease Cancer research |
| Zdroj: | Biochemical and Biophysical Research Communications. 351:192-197 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2006.10.020 |
| Popis: | MYCN amplification strongly correlates with unfavorable outcomes in patients with neuroblastoma. The aim of this study was to investigate the role of MYCN in neuroblastoma cell differentiation and apoptosis. We used the technique of RNA interference to inhibit MYCN gene expression in neuroblastoma cells with variable expression of MYCN. Our results showed that inhibition of MYCN gene expression in MYCN amplified cells induced apoptosis and suppressed cell growth; neuronal differentiation also occurred after MYCN gene silencing. Moreover, N-myc downregulation was associated with decreased Bcl-xL protein levels and caspase-3 activation. These data show that small interfering RNA directed to MYCN, which plays a crucial role in neuroblastoma cell survival, may provide a potential novel therapeutic option for aggressive neuroblastomas. |
| Databáze: | OpenAIRE |
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