Identification of SNP inHSP90AB1and its Association with the Relative Thermotolerance and Milk Production Traits in Indian Dairy Cattle
| Autor: | Rajib Deb, S. K. Bal, Umesh Singh, Sushil Kumar, Avinash V. Nirmale, M. P. Brahmane, Basavaraj Sajjanar, Paramjit Singh Minhas |
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| Rok vydání: | 2014 |
| Předmět: |
Genetics
HSP90AB1 Bioengineering Single-nucleotide polymorphism Biology Polymorphism Single Nucleotide Body Temperature Genotype frequency Milk Animal science Heat shock protein Genotype Animals SNP Cattle Female Animal Science and Zoology HSP90 Heat-Shock Proteins Genetic Association Studies Dairy cattle Biotechnology Genetic association |
| Zdroj: | Animal Biotechnology. 26:45-50 |
| ISSN: | 1532-2378 1049-5398 |
| DOI: | 10.1080/10495398.2014.882846 |
| Popis: | Heat shock proteins (Hsp) play crucial role in cellular thermotolerance and heat stress response. In the present work, Allele specific PCR (AS-PCR) was standardized to detect the nucleotide polymorphism within the HSP90AB1 gene (SNP g.4338TC) in Indian breeds of dairy cattle. The identified genotypes were associated with relative thermotolerance in terms of physiological parameters and milk production traits. The results of the experiments revealed that the genotype frequency of CC, CT, and TT for Sahiwal were 0.05, 0.78, and 0.17, respectively, and in Frieswal, the frequencies were 0.20, 0.70, and 0.10, respectively. The average rectal temperature (ART) and average respiration rates (ARR) were recorded during peak summer stress and heat tolerance coefficient (HTC) was calculated. The association studies indicated that TT genotypes had significantly (P0.01) higher HTC and lower ARR values than CT and CC in both the breeds. The TT genotype animals also had better production parameter in terms of total milk yield (TMY) (P0.01). These findings may partly suggest the role of HSP90AB1 polymorphisms in the regulation of heat stress response and consequent effect on production traits. Nevertheless, involvement of other regulatory mechanisms cannot be overruled. |
| Databáze: | OpenAIRE |
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