Structure and organization of the human Theta-class glutathione S-transferase and d-dopachrome tautomerase gene complex
| Autor: | Marjorie Coggan, Lel Whitbread, Angela T. Whittington, Philip G. Board |
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| Rok vydání: | 1998 |
| Předmět: |
Sequence analysis
Inverted repeat Pseudogene Molecular Sequence Data Biology Polymerase Chain Reaction Biochemistry Exon Humans Amino Acid Sequence Cloning Molecular Molecular Biology Gene Glutathione Transferase Genetics Base Sequence Intron DNA Exons Cell Biology Molecular biology Introns Intramolecular Oxidoreductases Glutathione S-transferase Multigene Family RNA splicing biology.protein Polymorphism Restriction Fragment Length Pseudogenes Research Article |
| Zdroj: | Biochemical Journal. 334:617-623 |
| ISSN: | 1470-8728 0264-6021 |
| DOI: | 10.1042/bj3340617 |
| Popis: | The structure and organization of the human Theta-class glutathione S-transferase (GST) genes have been determined. GSTT1 and GSTT2 are separated by approx. 50 kb. They have a similar structure, being composed of five exons with identical exon/intron boundaries. GSTT1 is 8.1 kb in length, while GSTT2 is only 3.7 kb. The GSTT2 gene lies head-to-head with a gene encoding d-dopachrome tautomerase (DDCT), which extends over 8.5 kb and contains four exons. The sequence between GSTT2 and DDCT may contain a bidirectional promoter. The GSTT2 and DDCT genes have been duplicated in an inverted repeat. Sequence analysis of the duplicated GSTT2 gene has identified an exon 2/intron 2 splice site abnormality and a premature translation stop signal at codon 196. These changes suggest that the duplicate gene is a pseudogene, and it has been named GSTT2P. |
| Databáze: | OpenAIRE |
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